IL 2 Rat

Interleukin-2 Rat Recombinant
Cat. No.
BT2140
Source
Escherichia Coli.
Synonyms

T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 97.0% as determined by SDS-PAGE and HPLC.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 16kDa.

The IL-2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 2 (IL2) is a cytokine that plays a crucial role in the proliferation of T and B lymphocytes, essential components of the immune system. The IL2 receptor is a complex structure consisting of multiple protein chains, with the gamma chain shared by other interleukins like IL4 and IL7. Interestingly, the expression of the IL2 gene in mature thymocytes, cells in the thymus gland where T cells mature, is monoallelic, meaning only one copy of the gene is expressed. This unusual regulatory mechanism highlights the importance of precise IL2 gene expression. Studies in mice have demonstrated that disrupting a similar gene to IL2 leads to conditions resembling ulcerative colitis, underscoring the crucial role of IL2 in mounting an effective immune response against foreign substances.
Description
Recombinant Rat Interleukin-2, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 135 amino acids. This protein, with a molecular weight of 16kDa, is purified using proprietary chromatographic methods, ensuring its high quality and purity.
Physical Appearance
The product appears as a colorless solution, free from any particles or cloudiness, after undergoing sterile filtration.
Formulation
The IL-2 solution is supplied as a 0.2µm filtered solution in a buffer containing 20mM PB at a pH of 3.5 and 30% Glycerol.
Stability
For optimal storage and to preserve its activity, the IL-2 solution should be stored at 4°C if used within 2-4 weeks. For longer storage durations, freezing at -20°C is recommended. The addition of a carrier protein, such as HSA or BSA at a concentration of 0.1%, is advised for long-term storage to maintain stability. It is crucial to avoid repeated cycles of freezing and thawing to prevent protein degradation.
Purity
The purity of the Interleukin-2 Rat Recombinant is exceptionally high, exceeding 97.0%. This high level of purity is determined using rigorous analytical techniques, including SDS-PAGE and HPLC.
Biological Activity
The biological activity of this product, a measure of its effectiveness, is determined by its ability to stimulate murine CTLL-2 cells. The ED50, representing the concentration at which half-maximal stimulation is observed, is less than 0.2ng/ml. This corresponds to a Specific Activity of 5,000,000IU/mg, indicating a highly potent product.
Synonyms

T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

Source
Escherichia Coli.
Amino Acid Sequence

APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ.

Product Science Overview

Structure and Function

Interleukin-2 is an O-glycosylated protein that consists of a four alpha-helix bundle structure. This structure is essential for its function in stimulating the growth and differentiation of various immune cells. IL-2 is particularly important for the proliferation of T cells, which are vital for the adaptive immune response. Additionally, IL-2 promotes the growth and differentiation of B cells, natural killer (NK) cells, lymphokine-activated killer cells, monocytes, macrophages, and oligodendrocytes .

Recombinant Production

Recombinant IL-2 from rats is typically produced using Escherichia coli (E. coli) as the expression host. The recombinant protein is often tagged with a His-tag to facilitate purification. The sequence of recombinant rat IL-2 usually spans from amino acid 21 to 155, with a calculated molecular weight of approximately 14.7 kDa .

Biological Activity

The biological activity of recombinant rat IL-2 is measured using cell proliferation assays, such as those involving CTLL-2 mouse cytotoxic T cells. The effective dose (ED50) for this activity is typically in the range of 0.04-0.2 ng/mL . This high level of activity underscores the potency of IL-2 as an immunoregulatory molecule.

Applications

Recombinant rat IL-2 is widely used in research to study immune responses and to develop therapeutic strategies for various diseases. It is particularly valuable in cancer research, where it is used to stimulate the immune system to target and destroy cancer cells. Additionally, IL-2 is used in studies of autoimmune diseases, infectious diseases, and transplantation immunology .

Storage and Stability

Recombinant rat IL-2 is typically supplied as a lyophilized powder and should be stored at -20°C to -80°C for long-term stability. Once reconstituted, the protein solution can be stored at 4-8°C for short-term use. It is important to avoid repeated freeze-thaw cycles to maintain the protein’s activity .

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