IL 2 Porcine

Interleukin-2 Porcine Recombinant
Cat. No.
BT2080
Source
Escherichia Coli.
Synonyms

T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin2 Porcine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15217 Dalton.
The IL-2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 2 (IL-2) is a cytokine that plays a crucial role in the immune system by promoting the growth and differentiation of T cells and B cells. These cells are essential components of the adaptive immune response, which targets specific pathogens. IL-2 exerts its effects by binding to a specific receptor complex on the surface of these lymphocytes. This receptor complex comprises multiple chains, including a gamma chain that is shared with receptors for other interleukins, namely IL-4 and IL-7. Interestingly, the expression of the IL-2 gene in mature thymocytes, which are T cell precursors, is monoallelic, meaning that only one copy of the gene is active. This unusual regulatory mechanism highlights the tight control exerted over IL-2 production. Underscoring its importance in immune function, studies in mice have shown that disrupting the IL-2 gene leads to severe inflammatory bowel disease resembling ulcerative colitis. This observation suggests that IL-2 is indispensable for maintaining immune homeostasis and preventing aberrant immune responses against harmless antigens.
Description
Recombinant Porcine Interleukin 2, expressed in E. Coli, is a purified protein with a molecular weight of 15,217 Daltons. It is a single, non-glycosylated polypeptide chain composed of 134 amino acids. The purification process involves proprietary chromatographic techniques to ensure high purity.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile-filtered.
Formulation
The protein was lyophilized from a concentrated (1 mg/ml) solution in 1x PBS at pH 7.4, which had been sterile-filtered through a 0.2 µm filter.
Solubility
To reconstitute the lyophilized IL-2, it is recommended to dissolve it in sterile distilled water to a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized IL-2 is stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it in a dry environment below -18°C. Once reconstituted, IL-2 should be stored at 4°C for 2-7 days. For extended storage, add a carrier protein like HSA or BSA (0.1%) to the solution and store it below -18°C. Avoid repeated freeze-thaw cycles to maintain protein stability.
Purity
The purity of IL-2 is greater than 96.0%, as determined by two independent analytical methods: Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) and Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of IL-2 is measured using a cell proliferation assay with murine CTLL-2 cells. The ED50, which represents the concentration of IL-2 required to achieve half-maximal cell proliferation, is less than 0.5 ng/ml. This corresponds to a specific activity greater than 2.0 × 106 IU/mg.
Synonyms

T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

Source
Escherichia Coli.
Amino Acid Sequence
APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT.

Product Science Overview

Structure and Function

IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex composed of three subunits: alpha (IL-2Rα), beta (IL-2Rβ), and gamma (IL-2Rγ). The gamma chain is also shared by other interleukins such as IL-4 and IL-7 . The binding of IL-2 to its receptor stimulates the growth, differentiation, and survival of antigen-selected cytotoxic T-cells, and it also promotes the development of regulatory T-cells, which are essential for maintaining immune tolerance and preventing autoimmune diseases.

Recombinant IL-2

Recombinant IL-2 is produced using genetic engineering techniques where the IL-2 gene is inserted into bacterial or yeast cells, which then express the IL-2 protein. This recombinant protein can be used for various research and therapeutic purposes. For instance, recombinant human IL-2 (rhIL-2) has been approved for the treatment of metastatic melanoma and renal cell carcinoma .

Porcine IL-2

Porcine IL-2 (pIL-2) is the IL-2 cytokine derived from pigs. It shares similar structural and functional characteristics with human IL-2 but is specific to porcine species. Recombinant porcine IL-2 is used in veterinary research and medicine to study immune responses in pigs and to develop treatments for porcine diseases. It is expressed in systems such as E. coli and P. pastoris .

Applications

Recombinant porcine IL-2 has several applications:

  • Research: It is used to study the immune system of pigs, including the regulation of T-cell activities and the development of immune responses.
  • Therapeutics: It can be used to enhance immune responses in pigs, particularly in the context of vaccines and treatments for infectious diseases.
  • Diagnostics: It is used in various assays to measure immune responses and to detect the presence of specific immune cells.

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