Interleukin-2, IL-2, T-cell growth factor, TCGF.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
IL2 Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (21-155 a.a) and having a molecular mass of 18.1 kDa.
IL2 Canine is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Interleukin-2, IL-2, T-cell growth factor, TCGF.
MGSSHHHHHH SSGLVPRGSH MGSHMAPITS SSTKETEQQM EQLLLDLQLL LNGVNNYENP QLSRMLTFKF YTPKKATEFT HLQCLAEELK NLEEVLGLPQ SKNVHLTDTK ELISNMNVTL LKLKGSETSY NCEYDDETAT ITEFLNKWIT FSQSIFSTLT.
The recombinant canine IL-2 protein consists of amino acids 21-155 of the canine IL-2 sequence and includes an N-terminal His-tag . The amino acid sequence of the recombinant protein is as follows :
MGSSHHHHHH SSGLVPRGSH MGSHMAPITS SSTKETEQQM EQLLLDLQLL LNGVNNYENP QLSRMLTFKF YTPKKATEFT HLQCLAEELK NLEEVLGLPQ SKNVHLTDTK ELISNMNVTL LKLKGSETSY NCEYDDETAT ITEFLNKWIT FSQSIFSTLT
The predicted molecular mass of the recombinant canine IL-2 protein is approximately 18.1 kDa . The protein is highly pure, with a purity greater than 95% as determined by SDS-PAGE . The endotoxin level is less than 1.0 EU per microgram of protein, as determined by the Limulus Amebocyte Lysate (LAL) method .
Recombinant canine IL-2 has been shown to stimulate cell cytotoxicity in the CTLL-2 mouse cytotoxic T cell line . The effective dose (ED50) range for this activity is less than or equal to 0.3 ng/mL . This demonstrates the protein’s ability to promote immune cell activation and proliferation, which is consistent with the known functions of natural IL-2 .
Recombinant canine IL-2 is used in various research applications to study the immune response and to develop potential therapeutic strategies for diseases involving the immune system . It may also be of use as an anti-tumor agent in cancer therapy, given its role in promoting the activation and proliferation of immune cells .
The recombinant canine IL-2 protein is typically formulated in phosphate-buffered saline (PBS) at pH 7.4 and is provided without preservatives . For short-term storage, the protein should be kept at 4°C, while for long-term storage, it should be aliquoted and stored at -20°C to avoid freeze-thaw cycles .