IL-1 Alpha Rat, His Active
Interleukin-1 alpha, IL-1 alpha.
Description
IL1A Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids (115-270 a.a) and having a molecular mass of 20.2kDa.
IL1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Interleukin-1 alpha, IL-1 alpha.
MGSSHHHHHH SSGLVPRGSH MGSSAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD
RIHLKAASLN DLQLEVKFDM YAYSSGGDDSKYPVTLKVSN TQLFVSAQGE DKPVLLKEIP
ETPKLITGSE TDLIFFWEKI NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS.
Product Science Overview
The recombinant form of rat IL-1α, tagged with a His-tag, is produced in Escherichia coli (E. coli). This recombinant protein is a single, non-glycosylated polypeptide chain containing 179 amino acids, with a molecular mass of approximately 20.2 kDa . The His-tag, consisting of 23 amino acids, is fused to the N-terminus of the protein, facilitating its purification through affinity chromatography techniques .
IL-1α is a potent mediator of the inflammatory response. It acts as an endogenous pyrogen, meaning it can induce fever by affecting the hypothalamus. Additionally, IL-1α stimulates the release of prostaglandins and collagenase from synovial cells, contributing to the inflammatory process . The biological activity of recombinant rat IL-1α has been measured in cell proliferation assays using D10.G4.1 mouse helper T cells, with an effective dose (ED50) of less than or equal to 20 pg/ml .
Recombinant IL-1α is widely used in research to study its role in inflammation and immune responses. It is also utilized in various assays, including enzyme-linked immunosorbent assays (ELISA) and cell proliferation assays, to investigate its biological functions and interactions with other cytokines and cellular receptors .
For optimal stability, recombinant rat IL-1α should be stored at 4°C if it will be used within 2-4 weeks. For longer-term storage, it is recommended to store the protein at -20°C, with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
