IL17A Mouse, Sf9
Sf9, Baculovirus cells.
IL17A, Ctla-8, Ctla8, IL-17, IL-17A, Il17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8.
Greater than 95% as determined by SDS-PAGE.
Description
IL17A Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 141 amino acids (26-158 a.a) and having a molecular mass of 16kDa.
IL17A is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin-17A, also known as IL17A, is a cytokine with a critical role in chronic and antimicrobial inflammation. Mice, rats, and humans share approximately 60% similarity in the amino acid sequence of IL17A. During bacterial infection, IL17A amplifies mucosal and epidermal inflammation, leading to increased chemokine production, neutrophil recruitment, and production of antibacterial proteins.
Produced in Sf9 insect cells using a baculovirus expression system, IL17A Mouse is a single, glycosylated polypeptide chain containing 141 amino acids (26-158 a.a) with a molecular weight of 16kDa. An 8 amino acid His tag is fused to the C-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.
The IL17A solution is provided at a concentration of 0.5mg/ml in a buffer consisting of 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity is determined by SDS-PAGE analysis and is consistently greater than 95%.
IL17A, Ctla-8, Ctla8, IL-17, IL-17A, Il17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8.
Sf9, Baculovirus cells.
AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAALEHHHHH H
Product Science Overview
Interleukin-17A (IL-17A) is a cytokine that plays a crucial role in chronic and antimicrobial inflammation. It is part of the interleukin-17 family, which includes six members (IL-17A to IL-17F). IL-17A is primarily produced by activated CD4+ T cells, also known as T helper 17 (Th17) cells. This cytokine is involved in various inflammatory diseases, including rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection .
The mouse recombinant IL-17A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 141 amino acids (26-158 a.a.) and has a molecular mass of approximately 16 kDa . The recombinant protein is fused to an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques . The amino acid sequence of the mouse IL-17A is as follows:
AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAALEHHHHH
IL-17A enhances mucosal and epidermal inflammation during bacterial infections by inducing the production of chemokines, which attract neutrophils to the site of infection . It also stimulates the production of antibacterial proteins. In human fibroblasts, IL-17A enhances the expression of intracellular adhesion molecule-1 (ICAM-1) and stimulates epithelial, endothelial, and fibroblastic cells to secrete other cytokines such as IL-6, IL-8, granulocyte-colony stimulating factor (G-CSF), and prostaglandin E2 (PGE2) .
Recombinant IL-17A is used in various research applications, including:
