IL12RB1 Human
Description
Product Specs
Product Science Overview
Interleukin 12 Receptor Beta 1 (IL-12Rβ1) is a crucial component of the immune system, playing a significant role in the regulation of cellular immunity. This receptor is part of the interleukin-12 (IL-12) receptor complex, which is essential for the differentiation of T helper cells into Th1 cells, a process vital for the body’s defense against intracellular pathogens.
IL-12Rβ1 is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily . It binds to IL-12 with low affinity and forms a high-affinity receptor complex when paired with IL-12Rβ2 . This receptor complex is responsible for mediating the biological activities of IL-12, including the induction of interferon-gamma (IFN-γ) production and the enhancement of cytotoxic activity in natural killer (NK) cells and T cells .
IL-12Rβ1 also associates with the IL-23 receptor (IL-23R) to form the IL-23 receptor complex, which is involved in IL-23 signal transduction. This interaction plays a role in the activation of the Jak-Stat signaling pathway, which is crucial for immune responses .
Recombinant human IL-12Rβ1 is produced using various expression systems, including Chinese Hamster Ovary (CHO) cells and mouse myeloma cell lines . The recombinant protein is typically purified to a high degree of purity (>95%) and is used in various research applications, including functional assays and binding studies .
The recombinant IL-12Rβ1 protein is often tagged with a His-tag or Fc region to facilitate purification and detection . It is available in both carrier-free and carrier-containing formulations, depending on the intended use. Carrier-free formulations are preferred for applications where the presence of carrier proteins, such as Bovine Serum Albumin (BSA), could interfere with experimental results .
Recombinant IL-12Rβ1 is widely used in research to study the IL-12 and IL-23 signaling pathways. It is employed in binding assays to measure the interaction between IL-12 or IL-23 and their receptors . Additionally, it is used in functional assays to investigate the biological activities mediated by these cytokines, such as the induction of IFN-γ production and the activation of NK cells and T cells .
