IL 12p75 Antibody

Interleukin-12 p75, Rat Anti-Mouse
Cat. No.
BT2648
Source
Synonyms
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Product Specs

Introduction
Interleukin-12 (IL-12) is a heterodimeric cytokine with a crucial role in cell-mediated immunity. It acts as a potent inducer of interferon-gamma (IFN-γ) production by T cells and natural killer (NK) cells, key players in the immune response against intracellular pathogens and tumor cells. Moreover, IL-12 drives the differentiation of naive T helper cells into the Th1 subset, which are characterized by their ability to secrete IFN-γ and promote cellular immune responses.
Formulation
The antibody is provided at a concentration of 1 milligram per milliliter (mg/ml) in phosphate-buffered saline (PBS) after reconstitution.
Shipping Conditions
To ensure stability during transportation, the antibody is shipped in lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, it is recommended to keep the lyophilized antibody at 4 degrees Celsius in a dry environment. After reconstitution, if not used within one month, aliquot the antibody solution and store it at -20 degrees Celsius to preserve its activity.
Solubility
To reconstitute the antibody, add sterile water (H2O) to the vial. Gently mix the solution by swirling or inverting the vial. Rinse the sides of the vial to ensure complete dissolution. Allow 30-60 seconds for the antibody to fully reconstitute before use.
Applications
This antibody is suitable for a variety of applications, including direct enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunoprecipitation.
Titer
In a direct ELISA, a 1:5,000 dilution of the antibody will result in an optical density (O.D.) of 0.5 when using an alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (IgG) secondary antibody from Jackson Laboratories.
Note
This antibody exhibits specificity for the intact IL-12 molecule. It does not bind to denatured forms of the p40 or p35 subunits individually.
Purification Method
Ion exchange.
Type
Rat Anti Mouse Monoclonal.
Clone
NYRmIL-12p75.
Immunogen
r.Murine IL-12 p75.
Ig Subclass
Rat IgG2b.

Product Science Overview

Role and Function

IL-12 plays a crucial role in the regulation of cell-mediated immune responses. It is a potent inducer of Th1 responses, leading to the production of interferon-gamma (IFN-γ) and interleukin-2 (IL-2) . These responses are essential for the body’s defense against intracellular pathogens and for the development of long-lasting immunity.

Rat Anti-Mouse IL-12 p75 Antibody

The Rat Anti-Mouse IL-12 p75 antibody, specifically the R2-9A5 monoclonal antibody, is designed to react with mouse IL-12 . This antibody has been shown to neutralize the biological effects of IL-12 when administered in vivo . It is commonly used in research to study the immune response and to investigate the role of IL-12 in various diseases.

Applications

The Rat Anti-Mouse IL-12 p75 antibody is used in various applications, including:

  • In vivo IL-12p75 neutralization: This involves administering the antibody to live animals to study the effects of IL-12 neutralization on immune responses .
  • ELISA: The antibody can be used in enzyme-linked immunosorbent assays to detect and quantify IL-12 levels in biological samples .
Preparation and Purification

The antibody is purified from cell culture supernatant in an animal-free facility using Protein G affinity chromatography . It is formulated in phosphate-buffered saline (PBS) at pH 8.0 and contains no stabilizers or preservatives . The endotoxin level is kept below 2 EU/mg, as determined by the Limulus Amebocyte Lysate (LAL) gel clotting assay .

Storage

The antibody solution should be stored at the stock concentration at 4°C and should not be frozen to maintain its stability and efficacy .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

IL 12p40 Antibody
Interleukin-12 p40, Rat Anti-Mouse
Cat. No.
BT2550
Source
IL12RB1 Human, Sf9
Interleukin 12 Receptor Beta 1 Human Recombinant, Sf9
Cat. No.
BT8680
Source
Sf9, Baculovirus cells.
IL 12 p35 Human
Interleukin-12 p35 Human Recombinant
Cat. No.
BT30496
Source
Escherichia Coli.
IL 12 p40 Human
Interleukin-12 p40 Human Recombinant
Cat. No.
BT30534
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.