IL 12 Mouse
Description
Interleukin-12 Mouse Recombinant produced in HEK 293 cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
The IL-12 is purified by proprietary chromatographic techniques.
Product Specs
p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS
WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS.
Product Science Overview
Mouse recombinant IL-12 is a heterodimer consisting of two subunits: p35 and p40. The p40 subunit is a 40 kDa protein, while the p35 subunit is a 35 kDa protein. Together, they form the active IL-12p70 heterodimer, which has a total molecular mass of approximately 70 kDa . The recombinant form of IL-12 is produced using various expression systems, such as HEK293 cells, to ensure high purity and biological activity .
IL-12 is a key cytokine in the immune response, with several important functions:
- Activation of NK Cells: IL-12 stimulates NK cells to produce IFN-γ, which enhances their cytotoxic activity against infected and malignant cells .
- T Cell Differentiation: IL-12 promotes the differentiation of naive CD4+ T cells into Th1 cells, which are essential for cell-mediated immunity .
- Cytokine Production: IL-12 induces the production of various cytokines, including IFN-γ, by T cells and NK cells .
- Enhancement of Cytotoxic Activity: IL-12 enhances the cytotoxic activity of CD8+ T cells and NK cells, contributing to the elimination of intracellular pathogens and tumor cells .
Recombinant mouse IL-12 is widely used in immunological research to study its effects on various immune cells and to investigate its potential therapeutic applications. Some common applications include:
- Cell Culture: IL-12 is used to stimulate and differentiate immune cells in vitro, providing insights into its role in immune responses .
- Functional Assays: IL-12 is employed in functional assays to assess its effects on cytokine production, cell proliferation, and cytotoxic activity .
- Animal Models: Recombinant IL-12 is used in animal models to study its therapeutic potential in diseases such as cancer, infectious diseases, and autoimmune disorders .
IL-12 has shown promise as a therapeutic agent due to its ability to enhance immune responses. It has been investigated in various clinical trials for its potential use in treating cancer, infectious diseases, and autoimmune disorders. However, its clinical application is limited by its potential side effects, such as systemic inflammation and toxicity .
In conclusion, Interleukin-12 (Mouse Recombinant) is a vital cytokine with significant roles in the immune system. Its recombinant form is a valuable tool in immunological research, providing insights into its functions and potential therapeutic applications.
