IL 7 Human, Yeast

Interleukin-7 Human Recombinant, Yeast
Cat. No.
BT6706
Source
Saccharomyces cerevisiae.
Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-7 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. The IL-7 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-7 (IL-7) is a cytokine crucial for the development of B and T cells. It partners with hepatocyte growth factor (HGF) to form a heterodimer that stimulates the growth of pre-pro-B cells. During early T cell development, IL-7 acts as a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB). Notably, intestinal epithelial and goblet cells can produce IL-7 locally, suggesting its regulatory role in intestinal mucosal lymphocytes. Studies with knockout mice models highlight IL-7's essential role in the survival of lymphoid cells.
Description
Recombinant human Interleukin-7, produced in yeast, is a single, glycosylated polypeptide chain composed of 152 amino acids. With a molecular weight of 17.4 kDa, it is purified using proprietary chromatographic techniques.
Physical Appearance
White, sterile-filtered, lyophilized powder.
Formulation
Lyophilized from a 1 mg/ml aqueous solution containing 20mM phosphate buffer.
Solubility
For reconstitution, dissolve the lyophilized Interleukin-7 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Interleukin-7 remains stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, store IL-7 at 4°C for 2-7 days. For long-term storage, freeze at -18°C after adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
Determined by SDS-PAGE analysis, the purity is greater than 98.0%.
Biological Activity
The ED50, determined by measuring the dose-dependent stimulation of thymidine uptake in the murine pre-B cell line 2E8, is less than 0.5 ng/ml. This corresponds to a specific activity greater than 2 x 106 units/mg.
Protein Content
Protein content is quantified using two independent methods: (1) UV spectroscopy at 280 nm, using an absorbance value of 0.418 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated using the PC GENE computer analysis program of protein sequences (IntelliGenetics). (2) Analysis by RP-HPLC, employing a standard solution of IL-7 as a reference standard.
Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Source
Saccharomyces cerevisiae.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu.

Product Science Overview

Biological Role and Functions

IL-7 plays a vital role in the proliferation, survival, and homeostasis of T and B cells. It stimulates the development of lymphoid progenitors and is essential for the growth of pre-B cells . IL-7 acts through its receptor, a heterodimer consisting of IL-7Rα (CD127) and the common γc chain (CD132), which is shared by other cytokine receptors like IL-2, IL-4, IL-9, IL-15, and IL-21 .

Recombinant IL-7

Recombinant human IL-7, produced in yeast, is used extensively in research and clinical settings. It is optimized for use in cell culture, functional assays, and differentiation studies. The recombinant form retains the biological activity of natural IL-7 and is crucial for various applications, including in vitro T cell expansion, T cell priming, and differentiation of T cells and iNKT cells .

Clinical Significance

IL-7 is indispensable for immune cell development and homeostasis. Deficiencies in IL-7 or its receptor can lead to severe immunodeficiency . Clinical trials of recombinant IL-7 have demonstrated its safety and potent immune reconstitution effects, making it a promising candidate for immune intervention strategies .

Production and Quality

Recombinant IL-7 is produced in yeast to ensure high purity and biological activity. It is sterile-filtered prior to lyophilization, with endotoxin levels generally below 0.1 ng/μg and purities above 95% . The biological activity is tested in appropriate bioassays, ensuring reproducible cell culture conditions without the need for time-consuming lot-to-lot testing .

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