The human recombinant IL-7 with a His tag is a non-glycosylated polypeptide chain containing 152 amino acids, with a molecular mass of approximately 21.97 kDa . The His tag, which consists of a sequence of histidine residues, is added to the N-terminus of the protein to facilitate purification through affinity chromatography . This recombinant protein is typically produced in Escherichia coli (E. coli) expression systems .
IL-7 is essential for the development of lymphoid progenitors and the proliferation of T and B cells . It acts as a growth factor for these cells and has anti-apoptotic properties, which help in the survival of naive and memory T cells . Additionally, IL-7 has been shown to have biological effects on natural killer (NK) cells and myeloid lineages .
Recombinant IL-7 is widely used in research and clinical applications. Some of its key applications include:
The recombinant IL-7 with a His tag is supplied as a sterile filtered clear solution, typically in phosphate-buffered saline (PBS) with 50% glycerol . For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it should be frozen at -20°C to maintain its stability .