IL7 Human, sf9
Description
IL7 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 161 amino acids (26-177 a.a.) and having a molecular mass of 18.4kDa.
IL7 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
ADPDCDIEGK DGKQYESVLM VSIDQLLDSM KEIGSNCLNN EFNFFKRHIC DANKEGMFLF RAARKLRQFL KMNSTGDFDL HLLKVSEGTT ILLNCTGQVK GRKPAALGEA QPTKSLEENK SLKEQKKLND LCFLKRLLQE IKTCWNKILM GTKEHHHHHH H
Product Science Overview
Interleukin-7 (IL-7) is a crucial cytokine involved in the development and homeostasis of the immune system. It plays a significant role in the proliferation and survival of T and B cells, making it essential for maintaining a functional immune response . Recombinant human IL-7, produced using the sf9 insect cell expression system, is widely used in research and therapeutic applications due to its high purity and biological activity .
Recombinant human IL-7 is typically produced using the baculovirus expression vector system (BEVS) in sf9 insect cells. This method involves the following steps:
- Gene Cloning: The human IL-7 gene is cloned into a baculovirus transfer vector.
- Transfection: The recombinant baculovirus is used to transfect sf9 insect cells.
- Protein Expression: The transfected sf9 cells express the IL-7 protein.
- Purification: The IL-7 protein is purified using chromatography techniques to achieve high purity and biological activity .
IL-7 is a member of the type I cytokine family and is primarily produced by non-hematopoietic stromal cells in the bone marrow, thymus, and other lymphoid organs . It acts as a growth factor for T cells and B cells, promoting their development, survival, and homeostasis. IL-7 also plays a critical role in the maintenance of naive and memory T cells by preventing apoptosis .
IL-7 exerts its effects through a heterodimeric receptor composed of the IL-7 receptor alpha chain (IL-7Rα) and the common gamma chain (γc), which is shared with other cytokine receptors such as IL-2, IL-4, IL-9, IL-15, and IL-21 . Binding of IL-7 to its receptor activates several intracellular signaling pathways, including the JAK-STAT and PI3K-AKT pathways, leading to cell proliferation, survival, and differentiation .
Recombinant human IL-7 has shown promise in various clinical applications, particularly in immune reconstitution and cancer immunotherapy. Clinical trials have demonstrated its safety and efficacy in enhancing immune responses in patients with immunodeficiency disorders and certain types of cancer . Additionally, IL-7 is being investigated for its potential to improve vaccine responses and treat chronic infections .
