IL 7 Mouse
Interleukin-7 Mouse Recombinant
Cat. No.
BT7142
Source
Escherichia Coli.
Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interleukin-7 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14.9kDa.
Product Specs
Introduction
Interleukin-7 (IL-7) is a cytokine crucial for the development of lymphoid cells, primarily secreted by stromal cells. It supports the growth and differentiation of precursor B cells and early T cells. Additionally, IL-7 exhibits biological effects on cells of the Natural Killer (NK) and myeloid lineages.
Description
Recombinant Mouse Interleukin-7, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 129 amino acids and has a molecular weight of 14.9 kDa.
Physical Appearance
Sterile white powder obtained by lyophilization (freeze-drying).
Formulation
The product is filtered through a 0.2µm filter and lyophilized. The initial solution for lyophilization is a 1mg/ml solution in phosphate-buffered saline (PBS) at pH 7.4 with 2% trehalose.
Solubility
To reconstitute the lyophilized Interleukin-7, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-7 remains stable for 3 weeks at room temperature. However, for long-term storage, it is recommended to store it in a dry environment below -18°C. After reconstitution, store IL-7 at 4°C for 2-7 days. For future use, store below -18°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 96.0%, as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED50, defined as the concentration that induces 50% of the maximum proliferation response, is determined by the dose-dependent stimulation of murine 2E8 cell proliferation. The ED50 is less than 0.2 ng/ml, corresponding to a specific activity greater than 5.0 x 106 IU/mg.
Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Source
Escherichia Coli.
Amino Acid Sequence
ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI.
Product Science Overview
Structure and Production
Mouse IL-7 is a recombinant protein produced in Escherichia coli (E. coli) and optimized for use in cell culture, differentiation studies, and functional assays . The recombinant form of mouse IL-7 typically includes a Met on the amino terminus and has a calculated molecular weight of approximately 15 kDa . The protein is purified to a high degree, with purities exceeding 95% and endotoxin levels below 0.01 ng/μg .
Biological Activity
IL-7 is primarily produced by non-hematopoietic stromal cells in the bone marrow, thymus, and lymphoid organs and tissues . It acts as both a T cell growth factor and a critical anti-apoptotic survival factor . The primary targets of IL-7 are T cells, B cells, and dendritic cells . It signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain .
Applications
Mouse IL-7 is widely used in various research applications, including:
- In vitro T cell expansion: It is used to expand naive T cells in culture .
- In vitro differentiation of T cells: It aids in the differentiation of invariant natural killer T (iNKT) cells .
- Investigation of IL-7 mediated signaling pathways: Researchers study the signaling pathways activated by IL-7 to understand its role in immune regulation .
Storage and Stability
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