IL 7 Antibody

Interleukin-7, Mouse Anti-Human
Cat. No.
BT7445
Source
Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7 TypeMouse Anti Human Monoclonal.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
Interleukin-7 (IL-7) is a crucial cytokine that plays a vital role in the development of B and T cells. It forms a heterodimer with hepatocyte growth factor (HGF), acting as a growth-stimulating factor for pre-pro-B cells. During early T cell development, IL-7 serves as a cofactor for the V(D)J rearrangement of the T cell receptor beta (TCRB). Produced locally by intestinal epithelial and goblet cells, IL-7 may function as a regulatory factor for lymphocytes within the intestinal mucosa. Studies involving knockout mice have indicated that IL-7 is essential for the survival of lymphoid cells.
Formulation
The antibody is supplied as a solution with a concentration of 1 milligram per milliliter in phosphate-buffered saline (PBS) after reconstitution.
Shipping Conditions
The antibody is shipped in a lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4 degrees Celsius in a dry environment. After reconstitution, if not used within a month, aliquot the antibody and store at -20 degrees Celsius.
Solubility
To reconstitute, add sterile water to the lyophilized antibody. Mix gently, ensuring the sides of the vial are washed, and allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for various applications, including Direct ELISA, Western Blot, Immuneprecipitation, Immunohistochemistry, and Intracellular staining.
Titer
In a direct ELISA assay, a 1:10,000 dilution of the antibody will yield an optical density (O.D) of 0.3 using alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (Jackson Laboratories) as the secondary antibody.
Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7 TypeMouse Anti Human Monoclonal.
Purification Method
Ion exchange.
Clone
NYRhIL7.
Immunogen
r.Human IL-7.
Ig Subclass
Mouse IgG1.

Product Science Overview

Mouse Anti-Human IL-7 Antibodies

Mouse anti-human IL-7 antibodies are monoclonal antibodies developed to target and neutralize human IL-7. These antibodies are used in various research and clinical applications to study the role of IL-7 in immune responses and to develop therapeutic strategies for diseases involving IL-7 dysregulation .

Production and Characteristics

Mouse anti-human IL-7 antibodies are typically produced by immunizing mice with human IL-7 protein. The resulting antibodies are then harvested and purified for use in experiments. These antibodies are highly specific to human IL-7 and can be used in techniques such as Western blotting, ELISA, immunohistochemistry, and flow cytometry .

Applications in Research and Therapy

In research, mouse anti-human IL-7 antibodies are used to investigate the biological functions of IL-7 and its role in various diseases. They are also employed in studies aimed at understanding the mechanisms of IL-7 signaling and its impact on immune cell development .

In therapeutic applications, these antibodies have potential use in treating diseases characterized by abnormal IL-7 activity. For example, they may be used to neutralize excess IL-7 in conditions such as autoimmune diseases or certain cancers where IL-7 contributes to disease progression .

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