IL5 Mouse, Sf9

Interleukin-5 (IL-5) is a cytokine that plays a crucial role in the growth and differentiation of B cells and eosinophils. It is a key regulator of eosinopoiesis, eosinophil maturation, and activation. Elevated production of IL-5 is associated with conditions such as asthma and hypereosinophilic syndromes .

IL-5 is a glycoprotein that belongs to the alpha-helical group of cytokines. Unlike other family members, IL-5 is present as a covalently linked antiparallel dimer . The recombinant mouse IL-5 protein is produced in Spodoptera frugiperda (Sf9) Baculovirus cells. This recombinant form is a single, glycosylated polypeptide chain containing 119 amino acids (21-133 a.a) and has a molecular mass of 13.9 kDa .

The recombinant IL-5 is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques . The protein is supplied as a sterile filtered colorless solution, formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol .

For short-term storage (2-4 weeks), the IL-5 solution can be stored at 4°C. For long-term storage, it is recommended to store the solution frozen at -20°C, with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent multiple freeze-thaw cycles .

The biological activity of recombinant mouse IL-5 is measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The effective dose (ED50) range is less than or equal to 1 ng/mL . This indicates the potency of IL-5 in promoting cell proliferation.

Recombinant mouse IL-5 is used extensively in laboratory research to study its role in immune responses, particularly in the context of eosinophil-mediated inflammation. It is also used to investigate the mechanisms underlying asthma and other eosinophilic disorders .