IGFBP 5 Human

Insulin-Like Growth Factor Binding Protein-5 Human Recombinant
Cat. No.
BT16415
Source
Escherichia Coli.
Synonyms
IGFBP-5, IBP-5, IGF-binding protein 5.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

IGFBP5 Human Recombinant produced in E.Coli is non-glycosylated homodimer containing 2x252 amino acids and having a molecular mass of 28.6kDa.
IGFBP5 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IGFBP5, a member of the insulin-like growth factor binding protein (IGFBP) family, is a protein containing an IGFBP domain and a thyroglobulin type-I domain. This protein interacts with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II to form a ternary complex. This complex circulates in the plasma, influencing the half-life of IGFs and their interactions with cell surface receptors. Different isoforms of IGFBP5 can be produced due to the presence of alternate transcriptional splice variants.
Description
Recombinant Human IGFBP5, produced in E. coli, is a non-glycosylated homodimer with a molecular weight of 28.6 kDa. Each homodimer consists of two polypeptide chains, each containing 252 amino acids. The purification of IGFBP5 is achieved using proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder that has been sterile filtered.
Formulation
IBP-5 was lyophilized from a solution of 1 mg/ml in 10 mM sodium citrate, pH 3.
Solubility
To reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized IBP5 can be stored at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. Once reconstituted, IGFBP5 should be stored at 4°C for 2-7 days. For prolonged storage, it is advisable to add a carrier protein (0.1% HSA or BSA) and store it below -18°C. Repeated freeze-thaw cycles should be avoided.
Purity
The purity is determined using the following methods, with a result exceeding 96.0%:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED50, which represents the concentration of IGFBP5 required to inhibit 50% of IGF-II induced proliferation of MCF-7 cells, is a measure of its biological activity. In the presence of 15 ng/ml of recombinant human IGF-II, the expected ED50 is less than 0.4 µg/ml. This corresponds to a specific activity greater than 2500 IU/mg.
Synonyms
IGFBP-5, IBP-5, IGF-binding protein 5.
Source
Escherichia Coli.
Amino Acid Sequence
LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE.

Product Science Overview

Introduction

Insulin-Like Growth Factor Binding Protein-5 (IGFBP-5) is a member of the insulin-like growth factor binding protein family, which plays a crucial role in regulating the activity of insulin-like growth factors (IGFs). IGFBP-5 is highly conserved across species and has a broad range of biological activities. The recombinant form of IGFBP-5, known as human recombinant IGFBP-5, is produced through recombinant DNA technology and is used in various research and therapeutic applications.

Structure and Function

IGFBP-5 was first identified and purified from human bone extracts and conditioned media collected from cultured human osteosarcoma cells . It has a high affinity for IGFs, particularly IGF-1 and IGF-2, and can modulate their bioavailability and activity. IGFBP-5 can prolong the half-life of IGFs in the circulation, inhibit IGF signaling by competing with the IGF-1 receptor for ligand binding, and potentiate IGF signaling by delivering IGFs to the IGF-1 receptor .

Biological Activities

IGFBP-5 is expressed in diverse cell types and its expression is regulated by various signaling pathways. It has both IGF-dependent and IGF-independent activities. In bone, IGFBP-5 stimulates osteoblast cell proliferation and bone formation. Studies have shown that recombinant human IGFBP-5 can increase osteoblast activity in vitro and enhance bone formation parameters in vivo . Additionally, IGFBP-5 has been shown to play a role in the survival and activation of hepatic stellate cells, which are involved in liver fibrosis .

Therapeutic Potential

The ability of IGFBP-5 to modulate IGF signaling and its broad range of biological activities make it a promising candidate for therapeutic applications. Recombinant human IGFBP-5 has been studied for its potential to enhance bone formation and treat bone-related disorders. It has also been investigated for its role in liver fibrosis and other diseases where IGF signaling is implicated .

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