IGFBP 4 Human

Insulin-Like Growth Factor Binding Protein-4 Human
Cat. No.
BT16328
Source
HEK293 cells.
Synonyms
Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a polyhistidine tag at the C-terminus. IGFBP4 predicted Mw is 27kDa and on SDS-PAGE appears as a 32kDa band under denaturing conditions.
IGFBP4 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Insulin-like growth factor-binding protein 4 (IGFBP-4) is a member of the insulin-like growth factor binding protein (IGFBP) family. It possesses both an IGFBP domain and a thyroglobulin type-I domain, enabling it to bind to insulin-like growth factors (IGFs) I and II. Present in the plasma in glycosylated and non-glycosylated forms, IGFBP-4, like other IGFBPs, can either hinder or enhance the biological activities of IGF or operate independently of IGF. Notably, IGFBP-4 stands out for its consistent inhibition of various cancer cells in vivo and in vitro, suggesting a potential role as an apoptotic factor. Its production is observed in all colon cancer cells, and its binding to IGFs prolongs their half-life and alters their interaction with cell surface receptors.
Description
This product is recombinant human IGFBP-4, encompassing amino acids Asp22 to Glu258. Produced in HEK293 cells, it features a C-terminal polyhistidine tag. The predicted molecular weight (Mw) of IGFBP4 is 27 kDa. However, under denaturing conditions on SDS-PAGE, it appears as a 32 kDa band. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, filtered, white lyophilized (freeze-dried) powder.
Formulation
Following extensive dialysis against phosphate-buffered saline (PBS), IGFBP 4 was lyophilized.
Solubility
To reconstitute the lyophilized IGFBP4, it is recommended to dissolve it in sterile 18M-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized IGFBP4 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, IGFBP-4 should be stored at 4°C for no longer than 2-7 days. For extended storage periods, the addition of a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is greater than 95.0% as determined by SDS-PAGE analysis.
Biological Activity
The ED50, measured by the ability to inhibit the biological activity of IGFII (20 ng/ml) on MCF7 human breast adenocarcinoma cells, ranges from 0.01 to 0.09 µg/ml.
Synonyms
Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP.
Source
HEK293 cells.
Amino Acid Sequence
DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE + His Tag.

Product Science Overview

Introduction

Insulin-Like Growth Factor Binding Protein-4 (IGFBP-4) is a member of the insulin-like growth factor binding protein (IGFBP) family. This family of proteins plays a crucial role in modulating the activity of insulin-like growth factors (IGFs), which are essential for growth and development in humans. IGFBP-4 is encoded by the IGFBP4 gene and is known for its ability to bind both IGF-I and IGF-II, thereby influencing their biological activities.

Structure and Expression

IGFBP-4 is a 24 kDa protein that circulates in the plasma in both glycosylated and non-glycosylated forms . It contains an IGFBP domain and a thyroglobulin type-I domain, which are critical for its binding affinity to IGFs . The protein is expressed in various tissues, including osteoblasts, epidermis, ovarian follicles, and the liver, where its mRNA expression is most abundant .

Function

The primary function of IGFBP-4 is to regulate the availability and activity of IGFs. By binding to IGF-I and IGF-II, IGFBP-4 prolongs their half-life and modulates their interaction with cell surface receptors . This binding can either inhibit or stimulate the growth-promoting effects of IGFs, depending on the cellular context . IGFBP-4 has been shown to play a role in various physiological processes, including cell growth, survival, and differentiation .

Mechanism of Action

IGFBP-4 exerts its effects by altering the interaction of IGFs with their receptors on the cell surface . This modulation can lead to either the inhibition or stimulation of IGF signaling pathways, which are involved in cell proliferation, differentiation, and apoptosis . The precise mechanism by which IGFBP-4 influences these pathways is still under investigation, but it is believed to involve complex interactions with other proteins and cellular components .

Clinical Significance

IGFBP-4 has been implicated in various diseases and conditions. For example, alterations in IGFBP-4 levels have been associated with growth disorders, cancer, and metabolic diseases . Understanding the role of IGFBP-4 in these conditions could provide valuable insights into potential therapeutic targets and strategies.

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