IFNG Monkey
Escherichia Coli.
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Interferon-gamma Rhesus Macaque Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 142 amino acid and having a molecular mass of approximately 16.8kDa.
IFNG is purified by proprietary chromatographic techniques.
Product Specs
Interferon-gamma (IFN-gamma) is a cytokine primarily released by activated lymphocytes, including T cells and natural killer cells, in response to specific antigens or mitogens. Beyond its antiviral properties, IFN-gamma plays a crucial role in regulating immune responses. It acts as a potent activator of macrophages, exhibits antiproliferative effects on transformed cells, and can enhance the antiviral and antitumor actions of type I interferons.
This product consists of recombinant Interferon-gamma (IFNG) from Rhesus Macaque, produced in E.Coli. It is a non-glycosylated polypeptide chain composed of 142 amino acids, with a molecular weight of approximately 16.8kDa. The purification of IFNG is achieved through proprietary chromatographic methods.
This product appears as a sterile, filtered, white lyophilized (freeze-dried) powder.
This product is lyophilized from a 0.2µm filtered concentrated solution in phosphate-buffered saline (PBS) at a pH of 7.4.
To reconstitute the lyophilized Interferon-gamma Rhesus Macaque, it is recommended to dissolve it in sterile 18MΩ-cm H₂O at a concentration not less than 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Lyophilized IFNG remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the desiccated product below -18°C. After reconstitution, Interferon-gamma Rhesus Macaque should be stored at 4°C for a period of 2-7 days. For extended storage, it should be kept below -18°C. It's crucial to avoid repeated freeze-thaw cycles to maintain product integrity.
The purity of this product is determined to be greater than 97.0% through the following analyses:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC).
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
The half-maximal effective concentration (ED50) of this product, as determined by an antiviral assay using human HeLa cells infected with encephalomyocarditis (EMC) virus, is less than 20.0 ng/ml. This corresponds to a specific activity greater than 5.0 x 10⁴ international units per milligram (IU/mg).
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Escherichia Coli.
QDPYVKEAEN LKKYFNAGDP DVADNGTLFL DILRNWKEES DRKIMQSQIV SFYFKLFKNF KDDQRIQKSV ETIKEDINVK FFNSNKKKRD DFEKLTNYSV TDSNVQRKAV HELIQVMAEL SPAAKIGKRK RSQMFRGRRA SQ.
Product Science Overview
Interferon-gamma (IFN-γ) is a critical cytokine in the immune system, known for its role in modulating immune responses and exerting antiviral, immunoregulatory, and anti-tumor properties. Recombinant Interferon-gamma derived from Rhesus Macaque (Macaca mulatta) is a valuable tool in biomedical research, particularly in studies involving non-human primate models.
Interferon-gamma is also referred to as type II or immune interferon. It is a dimeric protein composed of two identical subunits, each with a molecular weight of approximately 20-25 kDa. The protein is variably glycosylated, which can influence its stability and activity . IFN-γ is produced primarily by T lymphocytes and natural killer (NK) cells in response to antigenic or mitogenic stimuli.
The primary function of IFN-γ is to activate macrophages, enhance antigen presentation, and promote the differentiation of T helper cells into Th1 cells. It also plays a crucial role in the regulation of immune responses against intracellular pathogens, such as viruses and certain bacteria .
Recombinant Rhesus Macaque IFN-γ is produced using E. coli expression systems. The recombinant protein is typically purified to a high degree of purity (>97%) and is available in both carrier-free and carrier-containing formulations. The carrier protein, often Bovine Serum Albumin (BSA), is added to enhance protein stability and shelf-life .
Recombinant Rhesus Macaque IFN-γ is widely used in various research applications, including:
- Immunological Studies: It is used to study the immune responses in non-human primate models, particularly in the context of infectious diseases and vaccine development.
- Antiviral Assays: The antiviral activity of IFN-γ can be assessed using cell-based assays, such as those involving HeLa cells infected with encephalomyocarditis virus .
- Inflammatory Response Studies: IFN-γ is a key player in inflammatory responses, and its role can be studied in models of diseases such as COVID-19. For instance, in rhesus macaques, IFN-γ has been shown to drive the development of pulmonary lesions during SARS-CoV-2 infection .
