IFNG Human, His Active
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
IFNG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (24-161 a.a) and having a molecular mass of 18.4kDa. IFNG is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG.
Product Science Overview
Interferon-gamma (IFN-γ) is a dimerized soluble cytokine and the only member of the type II class of interferons. It plays a crucial role in the immune system by inhibiting viral replication directly and through its immunostimulatory and immunomodulatory effects . Human recombinant IFN-γ, particularly with a His tag, is a form of this cytokine that has been engineered for research and therapeutic purposes.
Recombinant human IFN-γ is typically produced in various expression systems, such as HEK293 cells or E. coli. The His tag, a sequence of histidine residues, is added to facilitate purification through affinity chromatography. This recombinant protein is often expressed as a single, non-glycosylated polypeptide chain containing 159 amino acids and having a molecular mass of approximately 18.4 kDa .
IFN-γ is known for its broad-spectrum antiviral properties. It is produced by lymphatic T cells and natural killer cells upon stimulation by antigens . The cytokine activates effector immune cells and enhances antigen presentation, making it a vital component in the body’s defense against infections and tumors . Recombinant IFN-γ retains these biological activities, making it a valuable tool in clinical research and therapeutic applications.
The production of recombinant human IFN-γ involves several steps:
- Gene Cloning: The gene encoding human IFN-γ is cloned into an expression vector.
- Transformation: The vector is introduced into a suitable host cell line, such as HEK293 or E. coli.
- Expression: The host cells are cultured under conditions that promote the expression of the recombinant protein.
- Purification: The His tag allows for easy purification of the protein using affinity chromatography techniques.
- Validation: The purity and biological activity of the recombinant protein are validated through various assays, such as SDS-PAGE and functional assays .
Recombinant human IFN-γ is used in various research and clinical applications, including:
- Antiviral Research: Studying the mechanisms of viral inhibition and developing antiviral therapies.
- Cancer Research: Investigating the role of IFN-γ in tumor suppression and developing immunotherapies.
- Immunology: Understanding the modulation of immune responses and the enhancement of antigen presentation .
