ICAM3 Human
Sf9, Baculovirus cells.
ICAM3, Intercellular adhesion molecule 3, ICAM-3, CD50, CDW50, ICAM-R, intercellular adhesion molecule 3 isoform 1
Greater than 90.0% as determined by SDS-PAGE.
Description
ICAM3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 698 amino acids (30-485 a.a) and having a molecular mass of 76.7kDa.
ICAM3 is fused to a 242 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
ICAM-3, also known as CD50, is a protein belonging to the intercellular adhesion molecule family. ICAM proteins are type I transmembrane glycoproteins that bind to the leukocyte adhesion molecule LFA-1. They typically possess 2-9 immunoglobulin-like C2-type domains. Expressed on all leukocytes, ICAM-3 serves as a primary ligand for LFA-1, playing a critical role in initiating immune responses. Beyond its adhesive properties, ICAM-3 also functions as a potent signaling molecule.
Recombinant Human ICAM3, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein consists of 698 amino acids (residues 30-485), resulting in a molecular weight of 76.7kDa. For purification purposes, ICAM3 is fused to a 242 amino acid hIgG-His-Tag at the C-terminus and is purified using proprietary chromatographic methods.
The ICAM3 solution is provided at a concentration of 0.25mg/ml in a buffer consisting of Phosphate-Buffered Saline (pH 7.4) and 10% glycerol.
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage to enhance stability. Repeated freezing and thawing of the product should be avoided.
The purity of the protein is determined by SDS-PAGE analysis and is guaranteed to be greater than 90%.
The biological activity of ICAM3 is assessed based on its ability to support the adhesion of HL-60 human promyelocytic cells. In this assay, immobilized human ICAM-3/CD50 is used to coat plates, and the adhesion of HL-60 cells is measured. The ED50, representing the concentration of ICAM-3 required for half-maximal cell adhesion, is typically less than or equal to 4ug/ml.
ICAM3, Intercellular adhesion molecule 3, ICAM-3, CD50, CDW50, ICAM-R, intercellular adhesion molecule 3 isoform 1
Sf9, Baculovirus cells.
ADLQEFLLRV EPQNPVLSAG GSLFVNCSTD CPSSEKIALE TSLSKELVAS GMGWAAFNLS NVTGNSRILC SVYCNGSQIT GSSNITVYRL PERVELAPLP PWQPVGQNFT LRCQVEDGSP RTSLTVVLLR WEEELSRQPA VEEPAEVTAT VLASRDDHGA PFSCRTELDM QPQGLGLFVN TSAPRQLRTF VLPVTPPRLV APRFLEVETS WPVDCTLDGL FPASEAQVYL ALGDQMLNAT VMNHGDTLTA TATATARADQ EGAREIVCNV TLGGERREAR ENLTVFSFLG PIVNLSEPTA HEGSTVTVSC MAGARVQVTL DGVPAAAPGQ PAQLQLNATE SDDGRSFFCS ATLEVDGEFL HRNSSVQLRV LYGPKIDRAT CPQHLKWKDK TRHVLQCQAR GNPYPELRCL KEGSSREVPV GIPFFVNVTH NGTYQCQASS SRGKYTLVVV MDIEAGSSHV EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH
Product Science Overview
ICAM-3 is a type I transmembrane glycoprotein that consists of five extracellular immunoglobulin-like domains, a hydrophobic transmembrane domain, and a short cytoplasmic tail . The protein is constitutively expressed on the surface of leukocytes, including T cells, B cells, macrophages, and dendritic cells .
The primary function of ICAM-3 is to mediate adhesion between cells by binding to specific integrin receptors such as LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) . This interaction is essential for the immune response, as it facilitates the initial contact between T cells and antigen-presenting cells, such as dendritic cells . Additionally, ICAM-3 plays a role in the clearance of apoptotic cells by attracting macrophages to phagocytose the dying cells .
Recombinant ICAM-3 is produced using recombinant DNA technology, where the ICAM3 gene is cloned and expressed in a suitable host cell system, such as a mouse myeloma cell line . The recombinant protein is then purified and characterized for use in various research and clinical applications .
One of the key applications of recombinant ICAM-3 is in studying cell adhesion mechanisms and immune cell interactions. It is also used in assays to investigate the role of ICAM-3 in various diseases, including inflammatory conditions and immune disorders .
ICAM-3 has been implicated in several diseases and conditions, including:
- Inflammatory Diseases: ICAM-3 is involved in the recruitment and activation of leukocytes at sites of inflammation .
- Immune Disorders: Abnormal expression or function of ICAM-3 can contribute to immune system dysregulation and autoimmune diseases .
- Cancer: ICAM-3 expression has been studied in various cancers, where it may play a role in tumor progression and metastasis .
In research, recombinant ICAM-3 is used to explore these roles and develop potential therapeutic strategies targeting ICAM-3 interactions.
