ICAM1 Mouse
Sf9, Baculovirus cells.
Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54 antigen, ICAM1, BB2, CD54, P3.58
Greater than 95.0% as determined by SDS-PAGE.
Description
ICAM1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 466 amino acids (28-485 a.a) and having a molecular mass of 51.2kDa.
ICAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Intercellular adhesion molecule 1 (ICAM1) is a protein that belongs to the ICAM family of proteins. This protein serves as a ligand for integrin alpha-L/beta-2, a leukocyte adhesion peptide. During leukocyte migration, ICAM1 facilitates the formation of endothelial apical assembly cups by activating ARHGEF26/SGEF & RHOG.
Recombinant Mouse ICAM1, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 466 amino acids (28-485 a.a), resulting in a molecular weight of 51.2kDa. The protein includes an 8 amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques.
The ICAM1 solution is provided at a concentration of 0.5mg/ml and is formulated in Phosphate-Buffered Saline (pH 7.4) with 10% glycerol.
The purity of the protein is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.
Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54 antigen, ICAM1, BB2, CD54, P3.58
Sf9, Baculovirus cells.
QVSIHPREAF LPQGGSVQVN CSSSCKEDLS LGLETQWLKD ELESGPNWKL FELSEIGEDS SPLCFENCGT VQSSASATIT VYSFPESVEL RPLPAWQQVG KDLTLRCHVD GGAPRTQLSA VLLRGEEILS RQPVGGHPKD PKEITFTVLA SRGDHGANFS CRTELDLRPQ GLALFSNVSE ARSLRTFDLP ATIPKLDTPD LLEVGTQQKL FCSLEGLFPA SEARIYLELG GQMPTQESTN SSDSVSATAL VEVTEEFDRT LPLRCVLELA DQILETQRTL TVYNFSAPVL TLSQLEVSEG SQVTVKCEAH SGSKVVLLSG VEPRPPTPQV QFTLNASSED HKRSFFCSAA LEVAGKFLFK NQTLELHVLY GPRLDETDCL GNWTWQEGSQ QTLKCQAWGN PSPKMTCRRK ADGALLPIGV VKSVKQEMNG TYVCHAFSSH GNVTRNVYLT VLYHSQNNVE HHHHHH
Product Science Overview
ICAM-1 is composed of 532 amino acids and has a molecular mass ranging from 90 to 110 kDa . The protein is heavily glycosylated and its extracellular domain is composed of multiple loops created by disulfide bridges . This structure allows ICAM-1 to bind to integrins, specifically CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1) . These interactions are essential for the adhesion and transmigration of leukocytes across the endothelium during immune responses .
ICAM-1 is typically expressed at low levels on the surface of endothelial cells and leukocytes. However, its expression can be significantly upregulated in response to pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor (TNF) . This upregulation enhances the ability of leukocytes to adhere to endothelial cells and migrate to sites of inflammation .
ICAM-1 is involved in various immune processes, including the activation of T-cells. It functions as a costimulatory molecule on antigen-presenting cells to activate MHC class II restricted T-cells, and on other cell types in association with MHC class I to activate cytotoxic T-cells . Additionally, ICAM-1 serves as a receptor for human rhinovirus, facilitating the entry of the virus into respiratory epithelial cells .
Recombinant ICAM-1, such as the mouse recombinant version, is produced using recombinant DNA technology. This involves inserting the gene encoding ICAM-1 into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. Recombinant ICAM-1 is used in various research applications, including studies on cell adhesion, immune response, and inflammation .
The recombinant form of ICAM-1 is widely used in research to study its role in immune responses and inflammatory processes. It is also utilized in the development of therapeutic agents targeting ICAM-1 interactions to treat inflammatory diseases and conditions involving excessive leukocyte adhesion .
