I TAC Mouse
C-X-C motif chemokine 11, I-TAC, Small-inducible cytokine B11, Cxcl11, Scyb11.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Product Specs
Chemokine (C-X-C motif) ligand 11 (CXCL11), also known as I-TAC, is a small cytokine that belongs to the CXC chemokine family. It is primarily expressed in leukocytes, pancreas, and liver, with moderate levels in the thymus, spleen, and lungs. Lower expression levels are found in the small intestine, placenta, and prostate. The expression of CXCL11 is significantly increased by interferon-gamma (IFN-γ) and interferon-beta (IFN-β), and slightly induced by interferon-alpha (IFN-α). I-TAC exerts its effects by binding to the CXCR3 chemokine receptor on target cells, exhibiting a stronger affinity compared to other ligands like CXCL9 and CXCL10. Notably, I-TAC acts as a chemoattractant for activated T cells. The gene encoding CXCL11 is situated on human chromosome 4, alongside several other members of the CXC chemokine family.
Avoid repeated freeze-thaw cycles.
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
C-X-C motif chemokine 11, I-TAC, Small-inducible cytokine B11, Cxcl11, Scyb11.
Product Science Overview
The gene encoding mouse CXCL11 is located on chromosome 5, in close proximity to the genes for IP-10 and MIG . The mouse CXCL11 cDNA encodes a precursor protein consisting of 100 amino acids, which includes a 21 amino acid signal peptide. This signal peptide is cleaved to produce the mature protein, which is 79 amino acids in length . The mature mouse CXCL11 shares 71% amino acid sequence identity with its human counterpart .
Mouse CXCL11 is expressed in various tissues, including the lung, heart, small intestine, and kidney, particularly during endotoxemia . Its expression is strongly induced by interferon-gamma (IFN-γ) and can be attenuated by glucocorticoid treatment . This chemokine is also upregulated in certain pathological conditions, such as AIDS, dementia, and multiple sclerosis .
CXCL11 plays a crucial role in the immune system by acting as a chemoattractant for activated T-cells. It binds to the CXC receptor 3 (CXCR3), which is predominantly expressed on activated T-cells . This interaction leads to the migration of T-cells to sites of inflammation, thereby contributing to the immune response.
Recombinant mouse CXCL11 is produced using an E. coli expression system . The recombinant protein is typically purified to a high degree of purity (≥ 98%) and is free from endotoxins . It is used in various research applications, including cell culture, ELISA, and functional assays to study its biological activity and interactions .
Recombinant mouse CXCL11 is widely used in immunological research to study T-cell migration and the role of chemokines in immune responses. It is also used in assays to investigate the signaling pathways activated by CXCR3 and to explore potential therapeutic targets for diseases involving immune dysregulation .
