I TAC Human, His
Description
I-TAC is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Interferon-inducible T-cell alpha chemoattractant (I-TAC), also known as CXCL11, is a small cytokine belonging to the CXC chemokine family. It is also referred to as Interferon-gamma-inducible protein 9 (IP-9). This chemokine plays a crucial role in immune responses by attracting activated T cells to sites of inflammation.
The gene encoding CXCL11 is located on human chromosome 4, along with other members of the CXC chemokine family . CXCL11 is highly expressed in peripheral blood leukocytes, pancreas, and liver, with moderate levels in the thymus, spleen, and lung. Lower expression levels are found in the small intestine, placenta, and prostate . The expression of CXCL11 is strongly induced by interferon-gamma (IFN-γ) and interferon-beta (IFN-β), and weakly induced by interferon-alpha (IFN-α) .
Human recombinant I-TAC (CXCL11) is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 94 amino acids (22-94) and has a molecular mass of approximately 10.6 kDa . The recombinant protein is fused to a 21 amino acid His-tag at the N-terminus, which facilitates purification using chromatographic techniques .
CXCL11 exerts its effects by interacting with the cell surface chemokine receptor CXCR3, with a higher affinity than other ligands for this receptor, such as CXCL9 and CXCL10 . It is chemotactic for activated T cells, meaning it directs the movement of these cells towards higher concentrations of the chemokine . This property is essential for the immune system’s ability to target and respond to sites of infection or inflammation.
Recombinant I-TAC (CXCL11) is used in various research applications, including studies on immune cell migration, inflammation, and cancer. It is also utilized in functional assays to investigate the chemotactic response of T cells and other immune cells . The protein is available in different quantities and is typically shipped with ice packs to maintain stability .
For short-term storage (2-4 weeks), I-TAC (CXCL11) should be kept at 4°C. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
