I TAC Human
Small inducible cytokine B11, CXCL11, I-TAC, IP-9, H174, Beta-R1, chemokine (C-X-C motif) ligand 11, IP9, b-R1, SCYB11, SCYB9B, MGC102770.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
I-TAC Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 8300 Dalton.
The I-TAC is purified by proprietary chromatographic techniques.
Product Specs
Chemokine (C-X-C motif) ligand 11 (CXCL11), also known as inducible T-cell alpha chemoattractant (I-TAC) and IP-9, is a small cytokine belonging to the CXC chemokine family. I-TAC is primarily found in peripheral blood leukocytes, pancreas, and liver, with moderate levels in the thymus, spleen, and lung. Low expression levels are observed in the small intestine, placenta, and prostate. The expression of the CXCL11 gene, responsible for encoding I-TAC, is significantly upregulated by IFN-g and IFN-b, and to a lesser extent by IFN-a. I-TAC exerts its effects on target cells by binding to the cell surface chemokine receptor CXCR3, exhibiting a higher affinity compared to other ligands for this receptor, namely CXCL9 and CXCL10. I-TAC exhibits chemotactic activity towards activated T cells. Notably, the CXCL11 gene is located on human chromosome 4, clustered with numerous other members of the CXC chemokine family.
Recombinant human I-TAC, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 73 amino acids. It has a molecular weight of 8.3 kDa. The purification of I-TAC is achieved through proprietary chromatographic techniques.
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Small inducible cytokine B11, CXCL11, I-TAC, IP-9, H174, Beta-R1, chemokine (C-X-C motif) ligand 11, IP9, b-R1, SCYB11, SCYB9B, MGC102770.
Product Science Overview
CXCL11 is a non-ELR CXC chemokine that consists of a 94 amino acid precursor protein. This precursor includes a 21 amino acid signal sequence, which is cleaved to form the mature 73 amino acid protein . The protein is highly expressed in peripheral blood leukocytes, pancreas, and liver, with moderate levels in the thymus, spleen, and lung . It is expressed at lower levels in the small intestine, placenta, and prostate .
The gene expression of CXCL11 is strongly induced by interferon-gamma (IFN-γ) and interferon-beta (IFN-β), and weakly induced by interferon-alpha (IFN-α) . CXCL11 functions as a chemoattractant for activated T cells by interacting with the chemokine receptor CXCR3 . It has a higher affinity for CXCR3 compared to other ligands such as CXCL9 and CXCL10 .
CXCL11 plays a crucial role in the immune response by mediating the chemotaxis of T cells. It is involved in various biological processes, including:
- Chemotaxis: The movement of cells towards the site of infection or inflammation .
- Positive regulation of leukocyte chemotaxis: Enhancing the movement of white blood cells to the site of infection .
- Chemokine-mediated signaling pathway: Facilitating communication between cells during immune responses .
- Response to lipopolysaccharide: Reacting to components of bacterial cell walls .
Recombinant human CXCL11/I-TAC is produced using Escherichia coli (E. coli) expression systems . The recombinant protein is typically purified to a high degree of purity (>97%) and is used in various research applications . It is often utilized to study its role in immune responses and its potential therapeutic applications.
