Introduction
Fatty acid binding protein 4, also known as FABP4 or aP2, is a protein belonging to the intracellular fatty acid binding protein (FABP) family. This family of proteins plays a crucial role in regulating the uptake, transport, and metabolism of fatty acids within cells. FABP4 is primarily found in adipocytes (fat cells) and macrophages (immune cells) and is involved in various metabolic processes, including insulin sensitivity, inflammation, and atherosclerosis. Its role in these processes has made it a potential therapeutic target for metabolic disorders.
Description
Human FABP4, derived from human adipose tissue, is a protein with a molecular weight of 14.587 kDa (excluding any glycosylation modifications) and consists of 131 amino acids.
Physical Appearance
The product appears as a white powder that has been filtered and lyophilized (freeze-dried).
Formulation
The FABP4 protein has been filtered through a 0.4 µm filter and lyophilized. It is supplied as a 0.5 mg/ml solution in a buffer containing 0.05 M phosphate and 0.075 M NaCl, with a pH of 6.5.
Solubility
To create a working solution, add deionized water to the lyophilized pellet to achieve an approximate concentration of 0.5 mg/ml. Allow the pellet to dissolve completely. The product is not sterile; therefore, it is crucial to filter it using a sterile filter of appropriate pore size before using it in cell culture.
Stability
The lyophilized protein should be stored at -20°C. After reconstituting the protein, aliquot it into smaller volumes to minimize freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a limited period; no significant changes are observed within two weeks at this temperature.
Purity
The purity of the protein is determined using SDS-PAGE analysis and is greater than 85.0%.
Synonyms
Fatty acid-binding protein adipocyte, AFABP, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, ALBP, A-FABP, FABP4.
Source
Human Adipose Tissue.
Amino Acid Sequence
CDAFVGTWKL VSSENFDDYM KEVGVGFATR KVAGMAKPNM IISVNGDVIT IKSESTFKNT EISFILGQEF DEVTADDRKV KSTITLDGGV LVHVQKWDGK STTIKRKRED DKLVVECVMK GVTSTRVYER A.
