FABP4 Protein

Fatty Acid Binding Protein 4 Human Recombinant
Cat. No.
BT6944
Source
Escherichia Coli.
Synonyms
Fatty acid-binding protein adipocyte, AFABP, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, ALBP, A-FABP, FABP4.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

14.7kDa protein containing 132 amino acid residues.

Product Specs

Introduction
Adipocyte fatty acid binding protein, FABP4, is a 15 kDa protein that belongs to the intracellular fatty acid binding protein (FABP) family. This family is known for its ability to bind fatty acids and related compounds, such as bile acids and retinoids, within an internal cavity. The expression of FABP4 in adipocytes is differentiation-dependent, and it plays a crucial role in regulating the biological functions of these cells. Studies involving targeted mutations in FABP4 in mice have shown significant protection against hyperinsulinemia and insulin resistance in both dietary and genetic obesity models. Adipocytes from FABP4-deficient mice exhibit reduced efficiency of ipolysis both in vitro and in vivo, and these mice also demonstrate moderate improvements in systemic dyslipidemia. Recent research has revealed FABP4 expression in macrophages upon differentiation and activation. In these cells, FABP4 influences inflammatory responses and cholesterol ester accumulation. Notably, total or macrophage-specific FABP4 deficiency provides substantial protection against atherosclerosis in apoE-/- mice. These findings collectively highlight the pivotal role of FABP4 in the development of key components of the metabolic syndrome through its distinct actions in adipocytes and macrophages.
Description
This protein consists of 132 amino acid residues and has a molecular weight of 14.7 kDa.
Physical Appearance
This product is supplied as a sterile filtered white powder that has been lyophilized (freeze-dried).
Formulation
This protein has been sterile filtered and lyophilized from a solution of 0.5 mg/ml in 0.05M Acetate buffer at pH 4.
Solubility
To reconstitute the lyophilized protein, dissolve it completely in 0.1M Acetate buffer at pH 4. For use at higher pH values, it is recommended to dilute the solution with the appropriate buffer to a concentration of 10 µg/ml. The solubility of this antigen is limited at higher concentrations.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated cycles of freezing and thawing. The reconstituted protein can be stored at 4°C for a limited time; it remains stable for up to two weeks at this temperature.
Purity
The purity of this protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Fatty acid-binding protein adipocyte, AFABP, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, ALBP, A-FABP, FABP4.
Source
Escherichia Coli.
Amino Acid Sequence
MCDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA.
Purification Method
Two-step procedure using size exclusion chromatography before and after refolding.
Specificity
The amino acid sequence of the recombinant human FABP4 is 100% homologous to the amino acid sequence of the human FABP4.

Product Science Overview

Introduction

Fatty Acid Binding Protein 4 (FABP4), also known as adipocyte-fatty acid-binding protein (A-FABP), is a member of the fatty acid-binding protein family. These proteins play a crucial role in the intracellular transport of fatty acids and other lipophilic substances. FABP4 is predominantly expressed in adipocytes and macrophages, where it is involved in various metabolic and inflammatory processes.

Structure and Function

FABP4 is a small, cytoplasmic protein that binds long-chain fatty acids and other hydrophobic ligands. The protein’s structure consists of a beta-barrel that forms a binding pocket for fatty acids. This binding facilitates the transport of fatty acids within cells, aiding in lipid metabolism and energy homeostasis .

Role in Metabolism

FABP4 is a key player in lipid metabolism. It regulates the storage and release of fatty acids in adipocytes and is involved in the development of insulin resistance and metabolic syndrome. Elevated levels of FABP4 are associated with obesity, type 2 diabetes, and cardiovascular diseases . In obese mice, deletion of FABP4 has been shown to improve insulin sensitivity and lipid metabolic disorders .

Involvement in Inflammation

Beyond its metabolic functions, FABP4 also plays a role in inflammation. It is secreted by adipocytes and macrophages and acts as an adipokine, influencing immune responses. FABP4 has been implicated in the development of atherosclerosis and other inflammatory conditions .

Clinical Significance

Due to its involvement in metabolic and inflammatory pathways, FABP4 is considered a potential biomarker for various diseases. Elevated serum levels of FABP4 have been linked to metabolic syndrome, non-alcoholic fatty liver disease (NAFLD), and cardiovascular diseases . Additionally, FABP4 is being explored as a therapeutic target for these conditions, with the aim of developing treatments that modulate its activity to improve metabolic health .

Human Recombinant FABP4

Human recombinant FABP4 is a laboratory-produced version of the protein, created using recombinant DNA technology. This allows for the production of large quantities of pure FABP4 for research and therapeutic purposes. Recombinant FABP4 is used in various studies to understand its function and role in disease, as well as in the development of potential therapeutic interventions .

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