FABP4 Human, His

Fatty Acid Binding Protein 4 Human Recombinant, His Tag
Cat. No.
BT6870
Source
Escherichia Coli.
Synonyms
Fatty acid-binding protein adipocyte, AFABP, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, ALBP, A-FABP, FABP4.
Appearance
Sterile Filtered colorless liquid formulation.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FABP4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 18 kDa. FABP4 is fused to His tag at N-terminus and purified by standard chromatography techniques.

Product Specs

Introduction
Adipocyte fatty acid binding protein FABP4, a 15 kDa protein, belongs to the intracellular fatty acid binding protein (FABP) family. This family is known for binding fatty acids and similar compounds (like bile acids or retinoids) within an internal cavity. The expression of FABP4 in adipocytes depends on their differentiation stage and plays a crucial role in regulating these cells' biological functions. Studies in mice show that targeted mutations in FABP4 offer significant protection against hyperinsulinemia and insulin resistance in both dietary and genetic obesity. Adipocytes lacking FABP4 exhibit reduced ipolysis efficiency both in vitro and in vivo, and these mice displayed moderately improved systemic dyslipidemia. Recent research also revealed FABP4 expression in macrophages during their differentiation and activation. In these cells, FABP4 influences inflammatory responses and cholesterol ester buildup. Notably, total or macrophage-specific FABP4 deficiency provides substantial protection against atherosclerosis in apoE-/- mice. These findings point to a central role of FABP4 in developing major metabolic syndrome components through its distinct actions in adipocytes and macrophages.
Description
Recombinant human FABP4, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 132 amino acids, resulting in a molecular weight of 18 kDa. The protein includes a His tag fused at the N-terminus and is purified using standard chromatography techniques.
Physical Appearance
The product is a clear, colorless liquid solution that has been sterilized by filtration.
Formulation
FABP4 His-Tag is supplied in a buffer solution containing 20mM Tris HCL (pH 8), 0.5mM DTT, and 50% glycerol.
Stability
For short-term storage (up to 4 weeks), the product should be kept refrigerated at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. Avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the FABP4 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Fatty acid-binding protein adipocyte, AFABP, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, ALBP, A-FABP, FABP4.
Source
Escherichia Coli.

Product Science Overview

Structure and Function

FABP4 is composed of a β-barrel structure that forms the lipid-binding site, and two α-helices that act as a lid on the β-barrel . This structure allows FABP4 to bind long-chain fatty acids and retinoic acid, facilitating their transport and metabolism within cells . The protein plays a crucial role in lipid transport, metabolism, insulin resistance, and angiogenesis .

Expression and Regulation

The expression of FABP4 is induced by long-chain fatty acids and peroxisome proliferator-activated receptor γ (PPARγ) agonists . It is involved in various metabolic processes, and its dysregulation is associated with several metabolic disorders. For instance, knockout studies in mice have shown that the absence of FABP4 prevents the development of atherosclerosis and increases insulin sensitivity .

Clinical Significance

Elevated levels of FABP4 have been observed in patients with conditions such as colorectal cancer and rheumatoid arthritis . This makes FABP4 a potential biomarker for these diseases. Additionally, its role in metabolic processes makes it a target for therapeutic interventions aimed at treating metabolic disorders.

Recombinant FABP4

Recombinant human FABP4, tagged with a His tag, is produced in Escherichia coli (E. coli) and is typically used in research applications such as ELISA, Western blot (WB), and immunoprecipitation (IP) . The His tag facilitates the purification of the protein using standard chromatography techniques . This recombinant protein retains the functional properties of the native protein, making it a valuable tool for studying the biological functions and interactions of FABP4.

Applications

Recombinant FABP4 is used in various research applications to study its role in lipid metabolism and its potential as a therapeutic target. It is also used in assays to investigate its binding properties and interactions with other molecules .

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