CD40 Human

CD40 Human Recombinant
Cat. No.
BT29517
Source
Escherichia Coli.
Synonyms
CD40 Molecule, TNF Receptor Superfamily Member 5, TNFRSF5, Tumor Necrosis Factor Receptor Superfamily, Member 5, Bp50, B-Cell Surface Antigen CD40, CD40L Receptor, CDW40, B Cell Surface Antigen CD40, B Cell-Associated Molecule, CD40 Antigen (TNF Receptor Superfamily Member 5), CD40 Type II Isoform, Nerve Growth Factor Receptor-Related B-Lymphocyte Activation Molecule, p50, Tumor Necrosis Factor Receptor Superfamily Member 5, CDw40, CD40 Antigen.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD40 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (21-193a.a) and having a molecular mass of 21.6kDa.
CD40 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD40 is a member of the TNF-receptor superfamily and plays a crucial role in various immune and inflammatory responses. These responses include T cell-dependent immunoglobulin class switching, memory B cell development, and the formation of germinal centers. The AT-hook transcription factor AKNA is believed to regulate the expression of both CD40 and its ligand, which is important for interactions between similar cells. The adapter protein TNFR2 interacts with CD40, acting as a mediator for signal transduction. The interaction between CD40 and its ligand is considered essential for microglial activation induced by amyloid-beta, marking it as an early event in the development of Alzheimer's disease.
Description
Recombinant human CD40, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 196 amino acids (21-193a.a) with a molecular weight of 21.6 kDa. It includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The CD40 protein solution is provided at a concentration of 0.25 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). Repeated freezing and thawing should be avoided.
Purity
The purity of the CD40 protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
CD40 Molecule, TNF Receptor Superfamily Member 5, TNFRSF5, Tumor Necrosis Factor Receptor Superfamily, Member 5, Bp50, B-Cell Surface Antigen CD40, CD40L Receptor, CDW40, B Cell Surface Antigen CD40, B Cell-Associated Molecule, CD40 Antigen (TNF Receptor Superfamily Member 5), CD40 Type II Isoform, Nerve Growth Factor Receptor-Related B-Lymphocyte Activation Molecule, p50, Tumor Necrosis Factor Receptor Superfamily Member 5, CDw40, CD40 Antigen.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEPPTACR EKQYLINSQC CSLCQPGQKL VSDCTEFTET ECLPCGESEF LDTWNRETHC HQHKYCDPNL GLRVQQKGTS ETDTICTCEE GWHCTSEACE SCVLHRSCSP GFGVKQIATG VSDTICEPCP VGFFSNVSSA FEKCHPWTSC ETKDLVVQQA GTNKTDVVCG PQDRLR

Product Science Overview

Structure and Function

CD40 is a 277 amino acid protein with a molecular weight of approximately 30 kDa. It consists of an extracellular domain, a single transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for binding to its natural ligand, CD40L (CD154), which is predominantly expressed on activated CD4+ T cells .

The interaction between CD40 and CD40L is crucial for various immune responses. This interaction leads to the activation and proliferation of B cells, differentiation of dendritic cells, and induction of cytokine production in peripheral blood monocytes and T cells . Additionally, CD40-CD40L signaling plays a significant role in T cell-APC interactions, B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis .

Recombinant Human CD40

Recombinant human CD40 (rhCD40) is a form of CD40 that is produced using recombinant DNA technology. This involves inserting the gene encoding CD40 into a suitable expression system, such as Escherichia coli (E. coli), to produce the protein in large quantities . Recombinant CD40 is used in various research applications, including cell culture, differentiation studies, and functional assays .

Applications

Recombinant human CD40 has several applications in scientific research:

  1. Stimulation of B Cell Activation and Proliferation: CD40-CD40L interaction is essential for B cell activation and proliferation. Recombinant CD40 can be used to study these processes in vitro .
  2. Dendritic Cell Maturation: CD40 signaling is crucial for the maturation of dendritic cells, which are key players in the immune response .
  3. Cytokine Production: CD40-CD40L interaction induces cytokine production in peripheral blood monocytes and T cells, which can be studied using recombinant CD40 .
  4. Cancer Immunotherapy: CD40-activated B cells have been studied as potent antigen-presenting cells that can potentially be used for cancer immunotherapy .

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