sCD40L Human, His
Description
CD40LG Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 169 amino acids (113-261 a.a.) and having a molecular mass of 18.3kDa.
The CD40LG is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Soluble CD40 Ligand (sCD40L), also known as Tumor Necrosis Factor-related Activation Protein (TRAP), is a recombinant protein that plays a crucial role in the immune system. It is a member of the tumor necrosis factor (TNF) superfamily and is primarily expressed on activated T cells . The recombinant form of this protein, tagged with a His tag, is widely used in research to study its biological functions and interactions.
The soluble CD40 Ligand/TRAP is produced as a recombinant protein in various expression systems, including E. coli and HEK 293 cells . The protein is typically fused with a His tag at the N-terminus to facilitate purification. The recombinant protein consists of 169 amino acids and has a molecular mass of approximately 18.3 kDa . The His tag allows for easy purification using nickel affinity chromatography, ensuring high purity and yield.
CD40 Ligand (CD40L) interacts with its receptor CD40, which is expressed on antigen-presenting cells (APCs) such as B cells, dendritic cells, and macrophages . This interaction is critical for various immune responses, including:
- B cell activation and differentiation: CD40L-CD40 interaction is essential for the activation of B cells, leading to their proliferation, differentiation, and antibody production .
- T cell-dependent humoral immunity: The interaction plays a pivotal role in T cell-dependent B cell responses, facilitating the formation of germinal centers and antibody affinity maturation .
- Inflammatory responses: CD40L is involved in the regulation of inflammatory pathways, contributing to the immune system’s ability to respond to infections and other threats .
CD40L is primarily expressed on activated CD4+ T lymphocytes . However, it is also found on other cell types, including platelets and endothelial cells . The expression of CD40L is tightly regulated and is induced upon T cell activation. Soluble CD40L (sCD40L) is generated through proteolytic cleavage of the membrane-bound form and can be detected in the circulation .
The expression and activity of CD40L are regulated at multiple levels:
- Transcriptional regulation: The gene encoding CD40L is regulated by various transcription factors that respond to immune signals .
- Post-translational modifications: CD40L undergoes post-translational modifications, including glycosylation and proteolytic cleavage, which influence its stability and activity .
- Interaction with receptors: CD40L binds to CD40 and other receptors, such as integrins, to mediate its effects on target cells .
Recombinant soluble CD40 Ligand/TRAP with a His tag is widely used in research to study its role in immune responses and its potential therapeutic applications. It is used in various assays, including:
- Binding studies: To investigate the interaction between CD40L and its receptors.
- Functional assays: To study the effects of CD40L on B cell activation, cytokine production, and other immune functions.
- Therapeutic research: To explore the potential of CD40L as a target for immunotherapy in diseases such as cancer and autoimmune disorders .
