sCD40L Mouse
Soluble CD-40 Ligand/TRAP Mouse Recombinant
Cat. No.
BT302
Source
Escherichia Coli.
Synonyms
CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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Description
sCD40 Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16409 Dalton.
The sCD40 is purified by proprietary chromatographic techniques.
The sCD40 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD40L, also known as CD154, is a surface protein found on T cells. It plays a crucial role in the immune response by interacting with CD40 on B cells. This interaction is vital for B cell activation, proliferation, and differentiation, leading to antibody production. Additionally, CD40L can stimulate other immune cells like monocytes, triggering them to release signaling molecules (cytokines) and enhance their ability to eliminate tumor cells. It can also promote the proliferation of activated T cells and induce the production of important cytokines like IFN-gamma, TNF-alpha, and IL-2.
Description
Recombinant Mouse sCD40, produced in E. coli, is a non-glycosylated polypeptide chain comprising 149 amino acids. With a molecular weight of 16409 Daltons, this protein represents the soluble form of CD40. It undergoes purification using proprietary chromatographic techniques to ensure its high quality.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a sterile solution containing 1mg/ml sCD40 and 10mM Sodium Phosphate buffer at pH 7.5.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized sCD40 in sterile 18MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized sCD40 remains stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, CD154 should be stored at 4°C and used within 2-7 days. For extended storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of sCD40 is greater than 98.0%, as determined by two methods: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of sCD40 was assessed by its ability to induce MIP-1 alpha and TNF-alpha production in mouse splenocytes. The ED50, which represents the concentration at which 50% of the maximal response is observed, was determined to be 0.1 µg/ml. This corresponds to a Specific Activity of 10,000 IU/mg.
Synonyms
CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Arg-Gly-Asp.
Product Science Overview
Structure and Expression
Biological Functions
CD40 Ligand binds to its receptor, CD40, which is found on antigen-presenting cells, platelets, and endothelial and epithelial cells . This interaction is critical for several immune responses:
- B Cell Activation: CD40 Ligand stimulates B cell proliferation and the secretion of all immunoglobulin isotypes in the presence of cytokines .
- Cytokine Production: It induces the production of cytokines such as IFN-gamma, TNF-alpha, and IL-2, which are essential for immune regulation .
- Tumoricidal Activity: CD40 Ligand has been shown to induce tumoricidal activity in peripheral blood monocytes .
Clinical Relevance
Research Applications
Recombinant soluble CD40 Ligand is widely used in research to study its effects on immune cells and its potential therapeutic applications. It is typically produced in E. coli and purified to high standards, with a purity of ≥ 98% by SDS-PAGE gel and HPLC analyses . The recombinant protein is used in functional assays to induce TNF-alpha and MIP-1alpha production by murine splenocytes .
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