CD40 Antibody, Biotin

CD40 is a 45-50 kDa type I transmembrane glycoprotein and a member of the tumor necrosis factor receptor (TNFR) superfamily . The CD40 molecule is primarily expressed on B cells, macrophages, dendritic cells, endothelial cells, and fibroblasts, with lower expression on plasma cells and a subset of peripheral T cells . The mouse anti-human CD40 antibody conjugated with biotin is a monoclonal antibody that specifically binds to the human CD40 antigen.

CD40 plays a crucial role in the immune system by mediating a variety of immune and inflammatory responses. It is involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis . The interaction of CD40 with its ligand, CD154 (also known as CD40L), is essential for T cell-B cell interactions and plays a significant role in co-stimulation and immune regulation .

CD40 is expressed on various cell types, including:

• B cells: Essential for B cell development and function.
• Macrophages and Dendritic Cells: Involved in antigen presentation and activation of T cells.
• Endothelial Cells and Fibroblasts: Contribute to inflammatory responses.
• Plasma Cells and Peripheral T Cells: Expressed at lower levels .

The primary functions of CD40 include:

• B Cell Activation: CD40 signaling is crucial for B cell activation, differentiation, and proliferation.
• Isotype Class-Switching: CD40 interaction with CD154 promotes isotype switching in B cells, allowing them to produce different classes of antibodies.
• Protection from Apoptosis: CD40 signaling helps protect B cells from apoptosis, ensuring their survival during immune responses .

CD40 exerts its effects through interaction with its ligand, CD154. This interaction triggers a cascade of signaling events that lead to the activation of various transcription factors and the production of cytokines. These signaling pathways are essential for the regulation of immune responses and the maintenance of immune homeostasis .

The expression and function of CD40 are tightly regulated by various factors, including cytokines and other signaling molecules. Dysregulation of CD40 signaling can lead to immune disorders and has been implicated in various diseases, including autoimmune diseases and cancers .

The mouse anti-human CD40 antibody conjugated with biotin is widely used in research applications, including:

• Flow Cytometry: For the detection and quantification of CD40 expression on various cell types.
• Immunohistochemistry (IHC): For the localization of CD40 in tissue sections.
• Functional Assays: To study the role of CD40 in immune responses and to investigate the effects of CD40 signaling on cell function .