CD40 Antibody, FITC

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50, and is a member of the tumor necrosis factor receptor (TNFR) superfamily . It is expressed on various cell types including B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors . CD40 plays a crucial role in the regulation of B cell development and maturation, immunoglobulin (Ig) isotype switching, and in protecting B cells from apoptosis induced by surface Ig .

The interaction of CD40 with its ligand, CD154 (also known as gp39), which is expressed on activated T cells, is essential for costimulation and immune regulation . This interaction is important for B cell proliferation and differentiation, as well as for the production of various cytokines .

FITC (Fluorescein Isothiocyanate) is a fluorescent dye commonly used in flow cytometry and other fluorescence-based techniques. When conjugated to antibodies, FITC allows for the visualization and quantification of specific cell surface markers . The FITC Mouse Anti-Human CD40 antibody is a monoclonal antibody that has been conjugated with FITC to enable the detection of CD40 expression on human cells .

The FITC Mouse Anti-Human CD40 antibody is widely used in research to study the roles of CD40 in immune responses, particularly in B cell biology . It is commonly used in flow cytometry to analyze the expression of CD40 on various cell types, including peripheral blood lymphocytes . This antibody is also useful for investigating the mechanisms of B cell activation, proliferation, and differentiation .

The FITC Mouse Anti-Human CD40 antibody should be stored undiluted at 4°C and protected from prolonged exposure to light . It is important to avoid freezing the antibody solution to maintain its stability and functionality .