sCD40L Mouse, sf9
Description
sCD40 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The soluble CD40 Ligand (CD40L), also known as CD154, TRAP (TNF-related activation protein), or TNFSF5, is a crucial protein in the immune system. It is a member of the tumor necrosis factor (TNF) superfamily and plays a significant role in immune responses by interacting with the CD40 receptor on various immune cells .
The CD40 Ligand is a type II transmembrane glycoprotein. The recombinant form of this protein, particularly the mouse version, is often produced using the sf9 insect cell expression system. This system is favored for its ability to produce high yields of properly folded and functional proteins .
The soluble form of CD40L is a 16.4 kDa protein containing 149 amino acid residues, which includes the receptor-binding TNF-like domain . This domain is essential for its interaction with the CD40 receptor, facilitating various immune responses.
CD40L is primarily expressed on the surface of activated T cells. Its interaction with the CD40 receptor on B cells, dendritic cells, and macrophages leads to several critical immune functions:
- B Cell Activation: CD40L stimulates B cell proliferation and differentiation, leading to the production of all immunoglobulin isotypes in the presence of cytokines .
- Cytokine Production: It induces the production of cytokines such as TNF-alpha, MIP-1alpha, IFN-gamma, and IL-2, which are vital for immune responses .
- Tumoricidal Activity: CD40L enhances the tumoricidal activity of peripheral blood monocytes, contributing to the body’s defense against cancer .
