CD40 Mouse
CD40 Mouse Recombinant
Cat. No.
BT29603
Source
Sf9,
Baculovirus cells.
Synonyms
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CD40.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CD40 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 180 amino acids (20-193 a.a.) and
having a molecular mass of 20.1kDa (Migrates at 18-28kDa on SDS-PAGE under
reducing conditions).
CD40 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD40, a member of the TNF receptor superfamily, plays a crucial role in immune responses and inflammation. It is essential for various immune processes such as T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The transcription factor AKNA regulates the expression of both CD40 and its ligand, highlighting their importance in cell-to-cell interactions. Notably, CD40 interacts with the adaptor protein TNFR2, which mediates downstream signaling. The CD40-ligand interaction has been implicated in amyloid-beta-induced microglial activation, suggesting its involvement in the early stages of Alzheimer's disease.
Description
Recombinant CD40, expressed in Sf9 insect cells, is a single, glycosylated polypeptide chain with 180 amino acids (20-193 a.a.) and a molecular weight of 20.1 kDa. Under reducing conditions, it migrates at 18-28 kDa on SDS-PAGE. The protein contains a 6-amino acid His tag at the C-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CD40 protein solution is provided at a concentration of 1.0 mg/ml in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein stability.
Purity
The purity of CD40 is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CD40.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
LGQCVTCSDK
QYLHDGQCCD LCQPGSRLTS HCTALEKTQC HPCDSGEFSA QWNREIRCHQ HRHCEPNQGL RVKKEGTAES
DTVCTCKEGQ HCTSKDCEAC AQHTPCIPGF GVMEMATETT DTVCHPCPVG FFSNQSSLFE KCYPWTSCED
KNLEVLQKGT SQTNVICGLK SRMRHHHHHH.
Product Science Overview
Structure and Function
CD40 is composed of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for binding to CD40L, which is expressed on activated T cells and platelets . The interaction between CD40 and CD40L plays a crucial role in the activation of both innate and adaptive immune responses .
The CD40/CD40L interaction is essential for various immune functions, including:
- B cell activation and proliferation: This interaction promotes the differentiation of B cells into plasma cells and memory B cells, leading to the production of antibodies .
- Dendritic cell maturation: CD40 signaling enhances the ability of dendritic cells to present antigens and activate T cells .
- Cytokine production: The interaction induces the production of various cytokines, which are critical for immune regulation .
Recombinant CD40 (Mouse)
Recombinant CD40 (Mouse) is a laboratory-produced version of the mouse CD40 protein. It is typically expressed in Chinese Hamster Ovary (CHO) cells to ensure proper folding and post-translational modifications . The recombinant protein is used in various research applications to study the CD40/CD40L interaction and its effects on immune responses.
Applications
Recombinant CD40 (Mouse) is widely used in immunological research to:
- Investigate immune cell interactions: Researchers use recombinant CD40 to study how CD40/CD40L interactions influence the behavior of immune cells .
- Develop immunotherapies: Understanding the CD40/CD40L pathway can lead to the development of novel immunotherapies for diseases such as cancer and autoimmune disorders .
- Study disease models: Recombinant CD40 is used in animal models to investigate the role of CD40 in various diseases, including infections, inflammatory conditions, and tumors .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
