CD34 Human
Description
Product Specs
Product Science Overview
CD34 is a 115 kDa glycosylated type I transmembrane protein . It contains a 31 amino acid signal sequence, a 259 amino acid extracellular domain (ECD), a 21 amino acid transmembrane sequence, and a 74 amino acid cytoplasmic domain . The extracellular domain of CD34 is heavily glycosylated, which is crucial for its function as a cell adhesion molecule .
CD34 is predominantly expressed on HSPCs and is used to identify and isolate these cells for clinical applications, such as bone marrow transplantation . It plays a critical role in the migration of HSPCs to specialized microvascular beds in the bone marrow that express vascular selectins (E- and P-selectin) . CD34-positive HSPCs exhibit E-selectin binding activity, which is essential for their homing and engraftment in the bone marrow .
The expression of CD34 on HSPCs makes it a valuable marker for enriching donor bone marrow with HSPCs prior to transplantation . CD34-positive cells are used in both autologous and allogeneic transplantation settings to reconstitute hematopoiesis following cytotoxic therapy for the treatment of malignant or autoimmune diseases . Additionally, CD34-positive HSPCs have been explored for their potential to transdifferentiate into non-hematopoietic cells, opening novel therapeutic avenues for treating diseases such as myocardial or cerebral infarction .
Recent studies have highlighted the potential of CD34 in various research areas. For instance, CD34-positive HSPCs have been shown to express functionally active receptors of neuromediators, suggesting a molecular interrelation between neuronal and hematopoietic signaling mechanisms . Furthermore, understanding the glycoforms of CD34 that discriminate normal HSPCs from leukemic cells could lead to new strategies for manipulating CD34-negative HSPC-enriched bone marrow or cord blood populations for clinical use .
In conclusion, CD34 is not just a marker for HSPCs but also plays a crucial role in their function and clinical applications. Ongoing research continues to uncover new aspects of CD34’s role in hematopoiesis and its potential therapeutic applications.
