CD34 Human, Sf9
Description
CD34 is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CD34 is a transmembrane phosphoglycoprotein that is heavily glycosylated. It is expressed on the surface of early hematopoietic and vascular-associated tissue. The protein is involved in cell adhesion, which is essential for the migration of HSPCs to the bone marrow. CD34 acts as a ligand for selectins, which are cell adhesion molecules that mediate the interaction between leukocytes and endothelial cells .
Hematopoiesis is the process by which all blood cells are produced from hematopoietic stem cells. CD34 is a critical marker used to enrich donor bone marrow with HSPCs before bone marrow transplantation. It is also used to identify and isolate HSPCs for research and clinical applications. CD34-positive HSPCs exhibit E-selectin binding activity, which is essential for their migration and homing to the bone marrow .
Recombinant CD34 (Human, Sf9) refers to the CD34 protein that has been produced using the Sf9 insect cell expression system. This system is commonly used for the production of recombinant proteins because it allows for proper protein folding and post-translational modifications, which are essential for the protein’s biological activity. The recombinant CD34 produced in Sf9 cells retains its functional properties and can be used in various research and clinical applications .
CD34-positive cells are widely used in clinical settings, particularly in bone marrow transplantation. The ability to isolate and enrich HSPCs using CD34 as a marker has significantly improved the outcomes of bone marrow transplants. Additionally, CD34-positive cells are being explored for their potential in regenerative medicine, including the treatment of myocardial infarction and other degenerative diseases .
In research, CD34 is used to study the biology of hematopoietic stem cells and their role in blood cell formation. It is also used to investigate the mechanisms of cell adhesion and migration, as well as the interactions between HSPCs and the bone marrow microenvironment. The recombinant CD34 protein produced in Sf9 cells provides a valuable tool for these studies, allowing researchers to explore the protein’s structure and function in detail .
