CD34 Human, GST

CD34 is a transmembrane protein with a highly glycosylated extracellular domain. It is involved in the early stages of hematopoiesis, the process by which blood cells are formed. CD34+ cells are often used as markers to identify and isolate hematopoietic stem cells (HSCs) for various therapeutic and research purposes.

The GST tag is a 26 kDa protein that is fused to the recombinant CD34 protein. This tag facilitates the purification of the recombinant protein using glutathione affinity chromatography. The GST tag also enhances the solubility and stability of the recombinant protein, making it more suitable for various experimental applications.

The recombinant CD34 protein with a GST tag is typically produced in Escherichia coli (E. coli) expression systems. The gene encoding CD34 is cloned into an expression vector that includes the GST tag sequence. This vector is then introduced into E. coli cells, which express the recombinant protein. The protein is subsequently purified using glutathione affinity chromatography, which exploits the high affinity between GST and glutathione.

1. Stem Cell Research: CD34+ cells are used to study the mechanisms of hematopoiesis and to develop stem cell-based therapies for various blood disorders.
2. Clinical Applications: CD34+ cells are used in bone marrow transplantation and regenerative medicine. The recombinant CD34 protein can be used to expand HSCs in vitro, enhancing their therapeutic potential.
3. Immunology: CD34 is used as a marker to identify and isolate various immune cell populations, including dendritic cells and endothelial progenitor cells.
4. Cancer Research: CD34+ cells are studied in the context of leukemia and other hematologic malignancies to understand the role of stem cells in cancer development and progression.