CD34 PAT3B6AT Antibody

CD34 Clone PAT3B6AT, Mouse Anti Human
Cat. No.
BT14225
Source
Synonyms

CD34 molecule, Hematopoietic progenitor cell antigen CD34, CD34 Antigen, CD34.

Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
CD34 is a transmembrane glycoprotein found on the surface of hematopoietic progenitor cells, which are precursors to various blood cells. This protein plays a crucial role in the development of blood cells from stem cells. Elevated levels of CD34 have been linked to increased angiogenesis, a process associated with tumor growth and potential relapse.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
This solution has a concentration of 1mg/ml and contains the following components: PBS with a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), keep refrigerated at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles to maintain product integrity.
Stability / Shelf Life
This product has a shelf life of 12 months when stored at -20°C and remains stable for 1 month at 4°C.
Applications
The CD34 antibody has undergone rigorous testing using various methods like ELISA, Western blot analysis, ICC/IF, and Flow cytometry. These tests confirm its specificity and reactivity. For optimal results, it is recommended to determine the optimal working concentration for each specific application.
Synonyms

CD34 molecule, Hematopoietic progenitor cell antigen CD34, CD34 Antigen, CD34.

Purification Method

CD34 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Type
Mouse Anti Human Monoclonal.
Clone

PAT3B6AT.

Immunogen

Anti-human CD34 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD34 amino acids 32-290 purified from E. coli.

Ig Subclass

Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Clone PAT3B6AT

Clone PAT3B6AT is a specific monoclonal antibody that targets the CD34 antigen. This clone is derived from mouse and is specifically designed to bind to human CD34. The monoclonal antibody is produced by immunizing mice with human CD34+ cells, followed by hybridoma technology to generate a stable cell line that produces the antibody.

Applications

The Mouse Anti-Human CD34 Clone PAT3B6AT is widely used in various scientific applications, including:

  • Flow Cytometry: This antibody is commonly used in flow cytometry to identify and isolate CD34+ cells from a mixed cell population. It helps in the analysis of hematopoietic stem cells and progenitor cells.
  • Immunohistochemistry: It is used to stain tissue sections to visualize the distribution and localization of CD34+ cells within the tissue.
  • Western Blot: The antibody can be used to detect CD34 protein levels in cell lysates or tissue extracts.
  • Immunocytochemistry: It is employed to stain cultured cells to study the expression and localization of CD34.
Significance in Research

The CD34 antigen is a critical marker for identifying hematopoietic stem and progenitor cells. It is extensively used in stem cell research, particularly in the isolation and characterization of stem cells for therapeutic purposes. The ability to identify and isolate CD34+ cells is essential for bone marrow transplantation, regenerative medicine, and cancer research.

Technical Details
  • Isotype: Mouse IgG1, κ
  • Reactivity: Human
  • Storage: The antibody should be stored undiluted at 4°C and protected from prolonged exposure to light. It should not be frozen to maintain its stability and functionality .

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