HLA-DRB1 Human

Major Histocompatibility Complex Class II DR Beta 1 Human Recombinant
Cat. No.
BT5308
Source
Escherichia Coli.
Synonyms
DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigen DRB1 16, DR-16, DR16, Human Leucocyte AntigenDRB1, MHC Class IIHLA-DR-Beta Cell Surface Glycoprotein, MHC Class IIHLA-DRw10-Beta.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HLA-DRB1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (30-227 a.a) and having a molecular mass of 25.2kDa.
HLA-DRB1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The protein known as HLA-DRB1, also called Major Histocompatibility Complex Class II DR Beta 1, belongs to the HLA class II beta chain paralogs. A heterodimer embedded in the membrane makes up Molecule class II, which consists of an alpha (DRA) and a beta chain (DRB). By displaying peptides derived from extracellular proteins, HLA-DRB1 plays a crucial role in the immune system. Antigen-presenting cells (APC), which include B lymphocytes, dendritic cells, and macrophages, express class II molecules. The beta chain is around 26–28 kDa in size. It is encoded by 6 exons, with exon one encoding the leader peptide, exons 2 and 3 encoding the two extracellular domains, exon 4 encoding the transmembrane domain, and exon 5 encoding the cytoplasmic tail.
Description
A single, non-glycosylated polypeptide chain comprising 219 amino acids (30-227 a.a), HLA-DRB1 Human Recombinant, generated in E. coli, has a molecular weight of 25.2 kDa. HLA-DRB1 is coupled to a 21 amino acid His-tag at the N-terminus and purified using specialized chromatographic methods.
Physical Appearance
A clear solution that has been sterile filtered.
Formulation
HLA-DRB1 protein solution at a concentration of 1 mg/ml in a buffer of 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
If the entire vial will be used within 2-4 weeks, store at 4°C. For longer storage periods, freeze at -20°C. It is advised to add a carrier protein, such as 0.1% HSA or BSA, for long-term storage. Avoid freeze-thaw cycles.
Purity
Greater than 85% purity as determined by SDS-PAGE analysis.
Synonyms
DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigen DRB1 16, DR-16, DR16, Human Leucocyte AntigenDRB1, MHC Class IIHLA-DR-Beta Cell Surface Glycoprotein, MHC Class IIHLA-DRw10-Beta.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAE YWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEV RWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK

Product Science Overview

Introduction

The Major Histocompatibility Complex (MHC) Class II DR Beta 1, also known as HLA-DRB1, is a protein-coding gene that plays a crucial role in the immune system. This gene is part of the MHC class II beta chain paralogs and is involved in presenting peptides derived from extracellular proteins to the immune system .

Structure and Function

The HLA-DRB1 gene encodes the beta chain of the MHC class II molecule, which is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB). The beta chain is approximately 26-28 kDa and is encoded by six exons. These exons encode different parts of the protein:

  • Exon 1: Leader peptide
  • Exons 2 and 3: Two extracellular domains
  • Exon 4: Transmembrane domain
  • Exon 5: Cytoplasmic tail

The beta chain contains all the polymorphisms that specify the peptide-binding specificities, making it essential for the immune response .

Role in the Immune System

HLA-DRB1 plays a central role in the immune system by presenting peptides derived from extracellular proteins. These peptides are displayed on the surface of antigen-presenting cells (APCs) for recognition by CD4-positive T cells. This interaction is crucial for initiating the immune response against pathogens .

Polymorphisms and Disease Associations

Hundreds of DRB1 alleles have been described, and some alleles are associated with increased susceptibility to certain diseases. For example:

  • DRB1*1302: Associated with acute and chronic hepatitis B virus persistence .
  • DRB1*1401: Increased frequency in Japanese patients with nonfamilial idiopathic cardiomyopathy .
Clinical Significance

The polymorphisms in the HLA-DRB1 gene have significant clinical implications. For instance, mismatching of HLA-DRB1 alleles between donor and recipient is associated with an increased risk of acute graft-versus-host disease (GVHD) after unrelated marrow transplantation . Additionally, certain HLA-DRB1 alleles are linked to autoimmune diseases such as multiple sclerosis and sarcoidosis .

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