HLA-DRB1 Human, Sf9
DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigen DRB1 16, DR-16, DR16, Human Leucocyte Antigen DRB1, MHC Class II HLA-DR-Beta Cell Surface Glycoprotein, MHC Class II HLA-DRw10-Beta.
Greater than 85.0% as determined by SDS-PAGE.
Description
HLA-DRB1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 207 amino acids (30-227a.a.) and having a molecular mass of 24.0kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). HLA-DRB1 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The HLA-DRB1 gene encodes for a protein known as Major Histocompatibility Complex Class II DR Beta 1. This protein plays a crucial role in the immune system by presenting peptides, which are fragments of proteins, to other immune cells. These peptides are derived from proteins found outside of the cell. HLA-DRB1 is a part of a group of proteins called HLA class II beta chain paralogs. The HLA class II molecule is made up of two chains, alpha (DRA) and beta (DRB), both of which are embedded in the cell membrane. This molecule is found on antigen-presenting cells (APCs) which include B lymphocytes, dendritic cells, and macrophages. The beta chain of HLA-DRB1 is approximately 26-28 kDa in size and is encoded by 6 exons. Exon 1 codes for the leader peptide, while exons 2 and 3 code for the two extracellular domains. Exon 4 codes for the transmembrane domain, and exon 5 codes for the cytoplasmic tail.
HLA-DRB1, a single, glycosylated polypeptide chain, is produced in Sf9 Baculovirus cells. This protein consists of 207 amino acids (30-227a.a.) and has a molecular weight of 24.0kDa. However, on SDS-PAGE, the molecular size appears to be approximately 28-40kDa. This discrepancy is due to glycosylation. The HLA-DRB1 protein is engineered with a 9 amino acid His tag at the C-terminus to aid in purification, which is carried out using proprietary chromatographic techniques.
The HLA-DRB1 protein solution is supplied at a concentration of 0.25mg/ml and is formulated in a buffer containing Phosphate Buffered Saline (pH 7.4) and 30% glycerol.
The purity of the HLA-DRB1 protein is determined by SDS-PAGE analysis and is found to be greater than 85.0%.
DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigen DRB1 16, DR-16, DR16, Human Leucocyte Antigen DRB1, MHC Class II HLA-DR-Beta Cell Surface Glycoprotein, MHC Class II HLA-DRw10-Beta.
ADPGDTRPRF LWQPKRECHF FNGTERVRFL DRYFYNQEES VRFDSDVGEF RAVTELGRPD AEYWNSQKDI LEQARAAVDT YCRHNYGVVE SFTVQRRVQP KVTVYPSKTQ PLQHHNLLVC SVSGFYPGSI EVRWFLNGQE EKAGMVSTGL IQNGDWTFQT LVMLETVPRS GEVYTCQVEH PSVTSPLTVE WRARSESAQS KHHHHHH
Product Science Overview
The Major Histocompatibility Complex (MHC) is a set of cell surface proteins essential for the acquired immune system to recognize foreign molecules in vertebrates, which in turn determines histocompatibility. The MHC Class II molecules are primarily expressed on antigen-presenting cells such as dendritic cells, macrophages, and B cells. Among these, the MHC Class II DR Beta 1 (HLA-DRB1) plays a crucial role in the immune response by presenting peptides derived from extracellular proteins.
The HLA-DRB1 gene encodes the beta chain of the MHC Class II molecule. This molecule is a heterodimer consisting of an alpha chain (encoded by the HLA-DRA gene) and a beta chain (encoded by the HLA-DRB1 gene). The beta chain is approximately 26-28 kDa and is encoded by six exons. Exon one encodes the leader peptide; exons two and three encode the two extracellular domains; exon four encodes the transmembrane domain; and exon five encodes the cytoplasmic tail .
The primary function of the MHC Class II molecules is to present processed antigens to CD4+ T helper cells. This antigen presentation is crucial for the activation of T cells, which in turn activate other immune cells to mount an effective immune response. The polymorphisms in the beta chain of the DR molecule specify the peptide-binding specificities, allowing the immune system to recognize a wide array of antigens .
The recombinant expression of HLA-DRB1 in Sf9 cells (Spodoptera frugiperda) is a common method used to produce large quantities of the protein for research purposes. Sf9 cells are insect cells that are often used in the baculovirus expression system, which is known for its high yield and proper folding of eukaryotic proteins.
The HLA-DRB1 gene is highly polymorphic, with hundreds of alleles described. Certain alleles are associated with increased susceptibility to various autoimmune diseases. For example, the HLA-DRB1*1501 allele is strongly associated with multiple sclerosis, while other alleles are linked to rheumatoid arthritis, type 1 diabetes, and other autoimmune conditions .
