HIV-1 p24, HRP

HIV-1 p24 Recombinant, HRP Labeled
Cat. No.
BT22530
Source
Escherichia Coli.
Synonyms
Appearance
Sterile filtered colorless clear solution.
Purity
Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived HRP recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 p24 immunodominant regions and fused to a GST tag at N-terminus.

Product Specs

Introduction
The human immunodeficiency virus (HIV) is a type of virus known as a retrovirus. It attacks the body's immune system, specifically the CD4+ T cells, also known as helper T cells. These cells play a crucial role in protecting the body from infections. Over time, HIV can destroy so many of these cells that the body becomes vulnerable to a variety of infections, a condition known as acquired immunodeficiency syndrome (AIDS). HIV is classified as a lentivirus, a subfamily of retroviruses characterized by their long incubation periods and ability to cause persistent infections. These viruses carry their genetic information in a single strand of RNA and are enveloped, meaning they are surrounded by a lipid membrane. Upon entering a host cell, HIV uses an enzyme called reverse transcriptase to convert its RNA into DNA. This viral DNA is then integrated into the host cell's DNA using another viral enzyme called integrase. Once integrated, the viral DNA can be used to produce new viral proteins and RNA, eventually leading to the assembly and release of new virus particles that can infect other cells.
Description
This product consists of a recombinant protein derived from Escherichia coli (E. coli). The protein is a non-glycosylated polypeptide chain containing the immunodominant regions of HIV-1 p24. It has been fused with a GST tag at the N-terminus and is purified to a high degree.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized through filtration.
Formulation
The product is supplied in a buffer containing 1.5M urea, 25mM Tris-HCl with a pH of 8.0, and 50% glycerol.
Purity
Analysis by HPLC and SDS-PAGE indicates that the purity of this product is greater than 90.0%.
Stability
While this product remains stable at 4°C for up to one week, it is recommended to store it at a temperature below -18°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain product integrity.
Applications
This product is suitable for use in various applications, including enzyme-linked immunosorbent assays (ELISA), Western blots, and flow-through assays.
Source
Escherichia Coli.

Product Science Overview

Introduction

The HIV-1 p24 protein is a major core protein of the Human Immunodeficiency Virus type 1 (HIV-1), which is the primary cause of the acquired immunodeficiency syndrome (AIDS). The p24 protein is a part of the Gag polyprotein, which is cleaved by viral protease during viral maturation to release p17, p24, and p12 proteins . The p24 protein is crucial for the assembly and maturation of the virus, making it a significant target for diagnostic and therapeutic strategies.

Recombinant HIV-1 p24 Protein

Recombinant p24 protein is produced using genetic engineering techniques, where the p24 gene is cloned and expressed in a host organism, such as Escherichia coli (E. coli). The recombinant protein is then purified using techniques like nickel affinity chromatography . This recombinant p24 protein retains its natural antigenic activity, making it useful for various applications, including diagnostic reagents and vaccine development .

HRP Labeling

Horseradish peroxidase (HRP) is an enzyme commonly used in biochemistry applications due to its ability to amplify signal detection. When the recombinant HIV-1 p24 protein is labeled with HRP, it allows for enhanced detection sensitivity in various assays, such as enzyme-linked immunosorbent assays (ELISA). The HRP-labeled p24 protein can specifically bind to antibodies against HIV-1, making it a valuable tool for detecting HIV infection .

Applications
  1. Diagnostic Assays: The HRP-labeled recombinant HIV-1 p24 protein is widely used in diagnostic assays to detect HIV infection. It can be used in ELISA to detect antibodies against HIV-1 in human serum and plasma . This is particularly useful for early diagnosis of HIV infection, as the p24 antigen can be detected before the appearance of antibodies.
  2. Research: The recombinant p24 protein is also used in research to study the immunogenicity and structure of the HIV-1 virus. It helps in understanding the virus’s life cycle and developing new therapeutic strategies .
  3. Vaccine Development: The antigenic properties of the recombinant p24 protein make it a potential candidate for multi-component HIV vaccine development. It can elicit an immune response, making it useful for vaccine research .

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THE BIOTEK
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