HIV-1 p24 Core
HIV-1 p24 Core Recombinant
Cat. No.
BT22188
Source
Escherichia Coli.
Synonyms
Appearance
Sterile filtered colorless clear solution.
Purity
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HIV-1 p24 Core is a 24kDa non-glycosylated polypeptide chain, containing the full length sequence of the HIV-1 p24.
Product Specs
Introduction
The human immunodeficiency virus, known as HIV, is a type of virus that attacks the body's immune system. Over time, HIV can weaken the immune system to the point where it can no longer fight off infections, leading to a condition called acquired immunodeficiency syndrome (AIDS). HIV specifically targets and infects crucial immune cells, including helper T cells (particularly CD4+ T cells), macrophages, and dendritic cells. These cells play a vital role in defending the body against infections. HIV infection leads to a decline in CD4+ T cells through various mechanisms, such as the direct killing of infected cells by the virus, increased programmed cell death (apoptosis) in infected cells, and the destruction of infected CD4+ T cells by CD8 cytotoxic lymphocytes that recognize the infected cells. HIV is classified as a lentivirus, belonging to the Retroviridae family. Lentiviruses share common structural characteristics and biological properties. These viruses are known for causing long-lasting illnesses with extended periods before symptoms appear. HIV's genetic material is in the form of RNA. When HIV enters a host cell, it uses an enzyme called reverse transcriptase to convert its RNA into DNA. This viral DNA is then integrated into the host cell's DNA using another enzyme called integrase. Once integrated, the viral DNA can be used to produce more viruses. After infecting a cell, HIV can either remain dormant (latent) within the cell or become active and replicate, releasing numerous new virus particles to infect other cells.
Description
HIV-1 p24 Core is a protein derived from the HIV-1 virus. It is 24 kilodaltons in size and does not contain any sugar modifications. This protein represents the full-length sequence of the HIV-1 p24 protein.
Physical Appearance
It is a clear solution without any particles, that is sterilized by filtration and appears colorless.
Formulation
It is formulated in a solution containing 50mM Tris buffer with a pH of 8.5 and 8M urea.
Purity
The purity of the protein is greater than 95%, as determined by High-Performance Liquid Chromatography (HPLC) analysis and SDS-PAGE.
Stability
HIV-1 p24 core, while stable at 4 degrees Celsius for one week, should ideally be stored at temperatures below -18 degrees Celsius. It is important to avoid repeated cycles of freezing and thawing.
Source
Escherichia Coli.
Specificity
Immunoreactive with all sera of HIV-1 infected individuals.
Product Science Overview
Introduction
The HIV-1 p24 core protein is a crucial component of the Human Immunodeficiency Virus type 1 (HIV-1), which is the primary cause of Acquired Immunodeficiency Syndrome (AIDS). The p24 protein is part of the Gag polyprotein, which is cleaved by viral protease during viral maturation to release matrix p17, core p24, and nucleocapsid proteins .
Structure and Function
Recombinant p24 Protein
Recombinant p24 protein is produced through genetic engineering techniques, where the p24 gene is cloned and expressed in a host organism, such as E. coli. This recombinant protein retains its natural antigenic activity, making it useful for various studies, including diagnostic reagents and multi-component HIV vaccine development .
Applications
- Diagnostic Reagents: The p24 antigen is a target for most diagnostic kits used for early detection of HIV infection. The fourth-generation test assays for HIV infection are based on p24 antigen detection, which helps in identifying HIV-infected individuals at an early stage .
- Vaccine Development: The recombinant p24 protein can elicit an immune response, making it a potential candidate for HIV vaccine development. It has been shown to induce the production of HIV p24 specific antibodies in animal models .
- Research: The recombinant p24 protein is used in various research applications, including studying the immunogenicity of the protein and its interactions with other viral components .
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