HIV-1 p24, 24 kDa
Sterile Filtered solution.
Greater than 93.0% as determined by SDS-PAGE.
Description
HIV-1 p24 recombinant is a full-length 231 amino acid recombinant protein having a molecular mass of 24 kDa.
Product Specs
Product Science Overview
The HIV-1 p24 protein is a crucial component of the Human Immunodeficiency Virus type 1 (HIV-1), which is the virus responsible for causing Acquired Immunodeficiency Syndrome (AIDS). The p24 protein is a capsid protein that forms the core of the virus, playing a vital role in its structure and function.
The HIV-1 p24 protein is derived from the Gag polyprotein precursor, which is initially synthesized as a 55 kDa protein. During the maturation process of the virus, the Gag polyprotein is cleaved by the viral protease to release several smaller proteins, including the matrix protein p17, the nucleocapsid protein, and the core protein p24 . The p24 protein itself has a molecular weight of approximately 24 kDa.
The p24 protein is indispensable for the reproduction of the HIV-1 virus. It constitutes the core of the virus particle and is involved in the assembly and maturation of the virus. The protein contains epitopes that prime helper CD4 T-cells, which are crucial for the immune response against the virus .
Recombinant HIV-1 p24 protein is produced using recombinant DNA technology, where the gene encoding the p24 protein is inserted into an expression system, such as Escherichia coli (E. coli). The recombinant protein is then purified to achieve high purity levels, typically greater than 90% .
The recombinant p24 protein is used in various research applications, including:
- SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis): A technique used to separate proteins based on their molecular weight.
- ELISA (Enzyme-Linked Immunosorbent Assay): A common laboratory technique used to detect and quantify antigens or antibodies.
- Western Blotting: A method used to detect specific proteins in a sample.
- Functional Studies: Research to understand the structure and function of the HIV-1 virus .
The recombinant HIV-1 p24 protein is widely used in HIV research. It serves as a standard in the titration of p24 antigens, which is essential for diagnosing and monitoring the progression of HIV infection. The protein is also used to study the immune response to HIV, as it can elicit lymphocyte proliferation and prime CD4 T-cells .
Additionally, the recombinant p24 protein is used in the development of diagnostic assays and vaccines. Its high purity and biological activity make it a valuable tool for researchers working to understand and combat HIV-1 .
