HIV-1 GAG
Greater than 78.0% as determined by SDS-PAGE.
Description
HIV-1 GAG Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain, encoded by the HIV gag gene, HXB2 (790-2292) and having a molecular mass of 55.0kDa. HIV-1 GAG is fused to a His-tag and is purified by proprietary chromatographic technique.
Product Specs
Human immunodeficiency virus (HIV) is a retrovirus that can weaken the immune system, increasing the risk of opportunistic infections. The virus primarily targets essential immune cells, including helper T cells (specifically CD4+ T cells), macrophages, and dendritic cells.
The Gag protein is crucial for HIV assembly. It's synthesized as a polyprotein within infected cells and comprises four functional segments. Gag alone can generate virus-like particles (VLPs) due to the assembly of approximately 2000 Gag molecules per virion. Gag proteins are involved throughout the virus's life cycle, from assembly and release to maturation into infectious virions. They also play a role in the events between the release of capsids into new cells and the integration of proviral DNA.
HIV-1 GAG Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain derived from the HIV gag gene, HXB2 (790-2292). It has a molecular mass of 55.0 kDa. The recombinant protein includes a His-tag for purification and is purified using a proprietary chromatographic technique.
Lyophilized with 1% glycerol.
Purity is determined to be greater than 78.0% by SDS-PAGE analysis.
To reconstitute lyophilized HIV-1 GAG, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. The reconstituted solution can be further diluted in other aqueous solutions as needed.
Lyophilized HIV-1 GAG, though stable at room temperature for up to 1 week, should be stored desiccated at a temperature below -18°C. After reconstitution, HIV-1 GAG can be stored at 4°C for 2-7 days. For long-term storage, it is recommended to freeze the solution at -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
This product is suitable for use in Western Blotting and SDS-PAGE applications.
Product Science Overview
The Human Immunodeficiency Virus type 1 (HIV-1) is the causative agent of Acquired Immunodeficiency Syndrome (AIDS). One of the key components of the HIV-1 virus is the Gag protein, which plays a crucial role in the virus’s life cycle. The Gag protein is encoded by the gag gene and is essential for virus assembly, maturation, and infectivity.
The Gag protein is initially synthesized as a precursor polyprotein known as Pr55Gag. This precursor undergoes proteolytic cleavage by the viral protease to produce several smaller proteins, including p17 (matrix), p24 (capsid), p7 (nucleocapsid), and p6 . These proteins are critical for the formation of the viral core and the assembly of new virions.
- p17 (Matrix): This protein is involved in targeting the Gag polyprotein to the plasma membrane, where virus assembly occurs.
- p24 (Capsid): The capsid protein forms the conical core of the virus, which encases the viral RNA genome.
- p7 (Nucleocapsid): This protein binds to the viral RNA and protects it during the assembly process.
- p6: The p6 protein is involved in the budding of new virions from the host cell membrane.
Recombinant HIV-1 Gag protein is a laboratory-produced version of the Gag protein. It is typically expressed in bacterial systems such as Escherichia coli to ensure high yield and purity . The recombinant protein retains the full-length sequence of the native Gag protein, making it a valuable tool for research and diagnostic purposes.
Recombinant HIV-1 Gag protein has several important applications in scientific research and medicine:
- Vaccine Development: The Gag protein is a target for vaccine development due to its essential role in the HIV-1 life cycle. Researchers use recombinant Gag protein to study immune responses and develop potential vaccine candidates.
- Diagnostic Tools: Recombinant Gag protein is used in diagnostic assays to detect HIV-1 infection. It serves as an antigen in enzyme-linked immunosorbent assays (ELISAs) and other immunoassays.
- Structural Studies: The recombinant protein is used in structural biology studies to understand the assembly and maturation of the HIV-1 virus. These studies provide insights into potential therapeutic targets.
- Antiviral Drug Development: By studying the interactions between Gag protein and other viral or host proteins, researchers can identify new targets for antiviral drugs.
