HCV NS3, Biotin
Description
The E.coli derived recombinant Biotin Labeled protein contains the HCV NS3 immunodominant regions, a.a. 1450-1643, 22 kDa. The Biotin labeled protein is fused with a 6xHis-Tag at N-terminus.
Product Specs
Product Science Overview
Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. It is a single-stranded positive-sense RNA virus that primarily targets the liver, leading to chronic hepatitis, cirrhosis, and hepatocellular carcinoma if left untreated . The HCV genome encodes a single polyprotein, which is processed into structural and nonstructural proteins, including the nonstructural protein 3 (NS3) .
The NS3 protein of HCV is a multifunctional enzyme with protease and helicase activities. It plays a crucial role in the viral life cycle by processing the viral polyprotein and unwinding RNA structures . The NS3 protein is essential for viral replication and is a target for antiviral drug development .
Recombinant NS3 protein is produced using genetic engineering techniques, where the NS3 gene is cloned and expressed in a host organism, such as Escherichia coli . The recombinant protein can be purified and used for various research and diagnostic purposes. One such variant is the NS3 protein tagged with biotin, which allows for easy detection and purification using streptavidin-based methods .
- Research: Recombinant NS3 protein is widely used in research to study the structure and function of the NS3 enzyme. It helps in understanding the mechanisms of viral replication and the development of antiviral drugs .
- Diagnostics: The NS3 protein is used in diagnostic assays to detect HCV infection. It serves as an antigen in enzyme-linked immunosorbent assays (ELISA) and other immunoassays .
- Vaccine Development: NS3 protein is a potential candidate for HCV vaccine development. DNA vaccines expressing NS3 protein have shown promise in inducing immune responses in preclinical studies .
