HCV NS3 His

Hepatitis C Virus NS3 Recombinant, His Tag
Cat. No.
BT14831
Source
Synonyms
Appearance
Purity
Protein is >95% pure as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The E.coli derived recombinant protein contains a total of 268 amino acids having a Mw of 27.9 kDa. The protein contains the HCV NS3 immunodominant regions, amino acids 1225-1456. The HCV NS3 protein is fused to a 36 His Tag at N-terminus and purified by standard chromatography techniques.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped, single-stranded RNA virus with a positive sense genome. It belongs to the Flaviviridae family. HCV replicates at a high rate, producing approximately one trillion particles daily in an infected individual. The virus's RNA polymerase lacks proofreading ability, resulting in a high mutation rate. This contributes to HCV's ability to evade the host's immune response. HCV is classified into six genotypes (1-6), each with several subtypes. The prevalence and distribution of these genotypes vary globally. Genotype determination is clinically significant as it influences the potential response to interferon-based therapy and the required treatment duration. Genotypes 1 and 4 show a lower response rate to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant HCV NS3 protein is derived from E. coli and consists of 268 amino acids, with a molecular weight of 27.9 kDa. It encompasses the HCV NS3 immunodominant regions, specifically amino acids 1225-1456. A 36 His tag is fused to the N-terminus of the protein to facilitate purification, which is achieved using standard chromatography techniques.
Purity
The purity of this protein is greater than 95% as determined by SDS-PAGE analysis.
Formulation
The HCV NS3 protein is supplied in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 10% glycerol.
Stability
For short-term storage, HCV-NS3 His is stable at 4°C for one week. However, for long-term storage, it is recommended to store the protein below -18°C. Repeated freeze-thaw cycles should be avoided.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT.

Product Science Overview

Introduction

Hepatitis C virus (HCV) is a significant global health concern, infecting millions of people worldwide. The virus can lead to chronic liver diseases, including cirrhosis and hepatocellular carcinoma. One of the critical components of HCV is the non-structural protein 3 (NS3), which plays a vital role in the virus’s replication and pathogenesis.

NS3 Protein

The NS3 protein of HCV is a multifunctional enzyme with both protease and helicase activities. The protease domain is responsible for cleaving the viral polyprotein into functional units, while the helicase domain unwinds RNA, which is essential for viral replication. Due to its crucial role in the viral life cycle, NS3 is a target for antiviral drug development .

Recombinant NS3 Protein

Recombinant NS3 protein is produced using genetic engineering techniques. The gene encoding the NS3 protein is cloned into an expression vector, which is then introduced into a host organism, typically Escherichia coli. The host organism expresses the NS3 protein, which can be purified for further study. The recombinant NS3 protein is often tagged with a polyhistidine (His) tag to facilitate purification using metal affinity chromatography .

His Tag

The His tag is a sequence of histidine residues added to the N- or C-terminus of a protein. This tag binds strongly to metal ions such as nickel or cobalt, allowing the tagged protein to be purified from a mixture of proteins using metal affinity chromatography. The His tag does not usually interfere with the protein’s function, making it a popular choice for protein purification .

Applications
  1. Drug Development: Recombinant NS3 protein is used in high-throughput screening assays to identify potential inhibitors of the NS3 protease and helicase activities. These inhibitors can serve as lead compounds for developing new antiviral drugs.
  2. Vaccine Development: The NS3 protein is also a candidate for vaccine development. Studies have shown that the NS3 protein can elicit an immune response, making it a potential component of an HCV vaccine.
  3. Diagnostic Tools: Recombinant NS3 protein can be used to develop diagnostic assays for HCV infection. These assays can detect antibodies against NS3 in patient samples, indicating an immune response to HCV .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

HCV Core NS3
Hepatitis C Virus Nucleocapsid (core) NS3 Recombinant
Cat. No.
BT12589
Source
Escherichia Coli.
HCV NS3
Hepatitis C Virus NS3 (1450-1643 a.a.) Recombinant
Cat. No.
BT13292
Source
HCV NS3 47.8kDa
Hepatitis C Virus NS3 47.8kDa Recombinant
Cat. No.
BT13793
Source
Escherichia Coli.
HCV NS3, HRP
Hepatitis C Virus NS3, Horseradish Peroxidase Recombinant
Cat. No.
BT14951
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.