HCV Core 24

Hepatitis C Virus Core 24 Recombinant
Cat. No.
BT11546
Source
Synonyms
Appearance
Purity
HCV Core 24 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The HCV Core 24 genotype-1b, E.coli derived recombinant protein, contains the HCV core nucleocapsid immunodominant regions. The protein is fused with b-galactosidase (114 kDa) at N-terminus.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped virus with a positive-sense, single-stranded RNA genome. It belongs to the family Flaviviridae. HCV displays a high replication rate, producing approximately one trillion viral particles daily in an infected individual. The virus's RNA polymerase lacks proofreading ability, leading to a high mutation rate, which contributes to its ability to evade the host's immune system. HCV is classified into six genotypes (1-6), each with several subtypes. The prevalence and distribution of these genotypes vary globally. Genotype determination is crucial clinically as it influences the potential response to interferon-based therapy and the required treatment duration. Genotypes 1 and 4 show a lower response rate to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
The HCV Core 24 protein is a recombinant protein derived from E. coli. It encompasses the immunodominant regions of the HCV core nucleocapsid of genotype 1b. This protein is expressed with an N-terminal fusion to b-galactosidase, resulting in a total molecular weight of 114 kDa.
Purity
The purity of the HCV Core 24 protein exceeds 95%, as determined by 10% SDS-PAGE followed by Coomassie blue staining.
Formulation
The HCV Core 24 protein is supplied in a buffer consisting of 20mM Tris-HCl (pH 8), 1.5M urea, and 10mM beta-mercaptoethanol.
Stability
For optimal stability, HCV Core 24 should be stored at -18°C. While it can remain stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Applications
The HCV Core 24 antigen is suitable for use in various research applications, including enzyme-linked immunosorbent assays (ELISA) and Western blots. It serves as an excellent antigen for the detection of HCV, exhibiting minimal cross-reactivity with other antigens.
Purification Method
HCV-Core 24 protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. It is an enveloped, positive-sense single-stranded RNA virus that belongs to the Hepacivirus genus within the Flaviviridae family . The virus’s genome encodes a single polyprotein, which is processed into structural and non-structural proteins essential for the virus’s life cycle .

Structure and Function of HCV Core Protein

The HCV core protein is one of the structural proteins encoded by the HCV genome. It plays a crucial role in the formation of the viral nucleocapsid, which is essential for the virus’s replication and assembly . The core protein is also involved in various host-cell interactions and has been implicated in the modulation of cellular processes such as apoptosis, lipid metabolism, and immune response .

Recombinant HCV Core 24

Recombinant HCV Core 24 refers to a specific segment of the HCV core protein that has been produced using recombinant DNA technology. This technology involves inserting the gene encoding the HCV core protein into an expression system, such as bacteria or yeast, to produce the protein in large quantities. The recombinant protein can then be purified and used for various research and diagnostic purposes .

Applications of Recombinant HCV Core 24
  1. Vaccine Development: Recombinant HCV Core 24 is used in the development of vaccines against HCV. It serves as an antigen to stimulate the immune system to produce antibodies against the virus, providing immunity to the host .
  2. Diagnostic Tools: The recombinant protein is used in diagnostic assays to detect the presence of antibodies against HCV in patient samples. This helps in the early diagnosis and management of HCV infections .
  3. Research: Recombinant HCV Core 24 is widely used in research to study the virus’s structure, function, and interactions with host cells. It helps in understanding the molecular mechanisms of HCV infection and the development of antiviral therapies .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

HCV Core 16.8kDa
Hepatitis C Virus Nucleocapsid (core) 16.8kDa Recombinant
Cat. No.
BT10965
Source
Escherichia Coli.
HCV Core 169aa
Hepatitis C Virus Nucleocapsid (core) 169aa Recombinant
Cat. No.
BT11092
Source
Escherichia Coli.
HCV Genotype 1b, 170 a.a.
Hepatitis C Virus Nucleocapsid (core) Genotype-1b, 170 a.a Recombinant
Cat. No.
BT12935
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.