HCV Core 22kDa, Rhodamine
Description
Product Specs
Product Science Overview
The Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. One of the key components of HCV research is the study of its core proteins, which play a crucial role in the virus’s structure and replication. The Hepatitis C Virus Core 22kDa, Rhodamine Recombinant is a recombinant protein used extensively in research to understand the virus’s biology and develop diagnostic and therapeutic tools.
The core protein of HCV is a structural protein that forms the viral nucleocapsid, which encases the viral RNA genome. This protein is approximately 22 kilodaltons (kDa) in size and is essential for the assembly and release of new viral particles. The core protein also interacts with host cell machinery, influencing various cellular processes and contributing to the pathogenesis of HCV infection .
Recombinant proteins are produced by expressing the gene of interest in a suitable host system, such as bacteria, yeast, or mammalian cells. In the case of the Hepatitis C Virus Core 22kDa, Rhodamine Recombinant, the core protein gene is typically expressed in Escherichia coli (E. coli) . This system is favored due to its simplicity, cost-effectiveness, and ability to produce large quantities of protein.
Rhodamine is a fluorescent dye commonly used in biological research for labeling and detecting proteins. The conjugation of rhodamine to the HCV core protein allows researchers to visualize and track the protein in various experimental setups. This conjugation is particularly useful in techniques such as fluorescence microscopy and flow cytometry, where the fluorescent signal can be used to study the protein’s localization, interactions, and dynamics within cells .
The Hepatitis C Virus Core 22kDa, Rhodamine Recombinant protein is used in a variety of research applications, including:
- SDS-PAGE: This technique is used to separate proteins based on their molecular weight, allowing researchers to analyze the purity and size of the recombinant protein .
- ELISA: Enzyme-linked immunosorbent assays (ELISA) are used to detect and quantify the presence of the core protein in samples, aiding in the development of diagnostic tests for HCV .
- Protein-Protein Interactions: The rhodamine-labeled core protein can be used to study interactions with other viral or host proteins, providing insights into the molecular mechanisms of HCV replication and pathogenesis .
