HBV AG-2 antibody

Hepatitis B Virus (AD & AY Antigens) AG-2 for Capture ELISA, Mouse antibody
Cat. No.
BT6881
Source
Synonyms
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Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Hepatitis B virus (HBV) is unusual among DNA viruses in that it uses reverse transcription during its replication cycle. HBV infects liver cells (hepatocytes), entering the cell through a receptor called sodium taurocholate cotransporting polypeptide (NTCP). Once inside the cell, the virus travels to the nucleus and converts its DNA into a closed circular form, which then serves as a template for the production of viral proteins and RNA. One of the RNA molecules produced is the pregenomic RNA (pgRNA), which serves as the template for reverse transcription. The pgRNA is packaged into new viral particles, and within these particles, it is reverse transcribed into DNA, generating new copies of the viral genome. These new viral particles can then go on to infect other cells.
Formulation
Supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS).
Shipping Conditions
Shipped at ambient temperature in lyophilized form.
Storage Procedures
Store lyophilized antibody at 4°C in a dry environment for long-term storage. For use after reconstitution, aliquot and store at -20°C if not intended for use within one month.
Solubility
Reconstitute with deionized water (H2O). Gently mix the solution, ensuring to wash the sides of the vial, and allow 30-60 seconds for complete reconstitution before use.
Applications
Suitable applications include direct enzyme-linked immunosorbent assay (ELISA), Western blotting, immunoprecipitation, and immunohistochemistry.
Titer
In direct ELISA against recombinant hepatitis B vaccine, a 1:10,000 dilution of the antibody produces an optical density (O.D.) of 0.5 when using alkaline phosphatase-conjugated rabbit anti-mouse immunoglobulin G (Jackson Laboratories).
Note
This antibody can detect both the AD and AY antigens in direct ELISA and is compatible with capture ELISA when used with an anti-hepatitis B surface antigen (HBsAg) antibody.
Purification Method
Ion exchange column.
Type
Mouse antibody Monoclonal.
Clone
NYRHepB-2.
Immunogen
r.Hep B vaccine (BTG).
Ig Subclass
mouse IgG1.

Product Science Overview

Introduction

Hepatitis B Virus (HBV) is a major global health concern, causing both acute and chronic liver diseases. The virus is characterized by its surface antigens, primarily the AD and AY subtypes, which are crucial for diagnostic and research purposes. The AG-2 mouse antibody is specifically designed for capture ELISA, a technique widely used to detect and quantify these antigens.

Hepatitis B Virus (HBV)

HBV is a DNA virus belonging to the Hepadnaviridae family. It primarily infects liver cells, leading to inflammation and potentially severe liver damage. The virus has several antigens, including the Hepatitis B surface antigen (HBsAg), which is a key marker for infection. The AD and AY subtypes of HBsAg are the most common and are used in various diagnostic assays .

AD & AY Antigens

The AD and AY antigens are subtypes of the Hepatitis B surface antigen (HBsAg). These antigens are part of the viral envelope and are crucial for the virus’s ability to infect host cells. The presence of these antigens in the blood is an indicator of active HBV infection. The AD subtype is more prevalent in certain geographical regions, while the AY subtype is more common in others .

Capture ELISA

Enzyme-Linked Immunosorbent Assay (ELISA) is a widely used technique for detecting and quantifying antigens and antibodies. In a capture ELISA, the target antigen is “captured” by a specific antibody coated on a microplate. The AG-2 mouse antibody is used in this context to capture the AD and AY antigens of HBV. This method is highly sensitive and specific, making it ideal for diagnostic and research applications .

AG-2 Mouse Antibody

The AG-2 mouse antibody is a monoclonal antibody specifically designed to bind to the AD and AY antigens of HBV. It is produced by immunizing mice with these antigens and then isolating the specific antibody-producing cells. The antibody is then purified and used in capture ELISA to detect the presence of HBV antigens in patient samples .

Applications

The AG-2 mouse antibody for capture ELISA is used in various applications, including:

  • Diagnostic Testing: Detecting HBV infection in patients by identifying the presence of AD and AY antigens in blood samples.
  • Research: Studying the epidemiology and pathogenesis of HBV by analyzing the distribution and prevalence of different HBsAg subtypes.
  • Vaccine Development: Evaluating the efficacy of HBV vaccines by measuring the immune response to different HBsAg subtypes .

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