HBV HBe

Hepatitis B Virus HBe Recombinant
Cat. No.
BT10058
Source
Synonyms
Appearance
Purity
HBV HBe protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.Coli derived recombinant protein contains the HBV HBe adw immunodominant region is fused to a GST tag and the Mw is 43,897.6 Dalton.

Product Specs

Introduction
Hepatitis B virus (HBV) is unusual amongst viruses as it replicates its DNA genome by reverse transcription. HBV initially enters the cell by binding to a receptor on the surface of a liver cell, called a hepatocyte. Following entry, the viral core, containing the DNA genome, is transported to the cell nucleus. Here, the partially double-stranded DNA in the virus particle is converted to a circular, double-stranded molecule (cccDNA) that acts as the template for viral gene expression. Amongst the viral gene products produced is a large RNA, the pregenomic RNA (pgRNA) that is both translated into viral proteins and also reverse transcribed to make more copies of the viral DNA genome. Newly synthesised viral DNA can either be packaged into progeny virus particles or be converted into more cccDNA, thereby increasing the copy number of viral DNA in the nucleus.
Description
The HBV HBe protein is a recombinant protein expressed in E. coli. The protein sequence contains the immunodominant region of the HBV HBe adw strain fused to a GST tag. The molecular weight of the fusion protein is 43.8 kDa.
Purity
The purity of the HBV HBe protein is greater than 95% as determined by 10% SDS-PAGE and Coomassie blue staining.
Formulation
The HBV HBe protein is supplied at a concentration of 1 mg/ml in a solution containing 50 mM Tris-HCl pH 8.5, 5 mM DTT, 10 mM beta-mercaptoethanol and 8 M urea.
Stability
The HBV HBe protein is stable at 4 °C for 1 week. For long-term storage, the protein should be stored at -18 °C or colder. Avoid repeated freeze-thaw cycles.
Purification Method
Purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera HBV-infected individuals.

Product Science Overview

Introduction

Hepatitis B virus (HBV) is a significant global health concern, causing both acute and chronic liver infections. One of the key proteins associated with HBV is the Hepatitis B e antigen (HBeAg), which plays a crucial role in the virus’s life cycle and its interaction with the host’s immune system. The recombinant form of HBeAg is used in various diagnostic and research applications to better understand and manage HBV infections.

Hepatitis B Virus (HBV)

HBV is a DNA virus belonging to the Hepadnaviridae family. It primarily infects liver cells (hepatocytes) and can lead to severe liver diseases, including cirrhosis and hepatocellular carcinoma. The virus has a complex structure, consisting of an outer lipid envelope and an inner nucleocapsid core containing the viral DNA and DNA polymerase.

HBeAg and Its Role

HBeAg is a secreted protein derived from the precore region of the HBV genome. It is not essential for viral replication but is considered an important marker for viral replication and infectivity. The presence of HBeAg in the blood of infected individuals indicates active viral replication and high infectivity. Conversely, the loss of HBeAg and the appearance of antibodies against HBeAg (anti-HBe) are associated with a lower level of viral replication and a better prognosis.

Recombinant HBeAg

Recombinant HBeAg is produced using recombinant DNA technology, which involves inserting the gene encoding HBeAg into an expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in various diagnostic assays to detect HBeAg in patients’ blood samples, helping to assess the stage and severity of HBV infection.

Applications of Recombinant HBeAg
  1. Diagnostic Assays: Recombinant HBeAg is widely used in enzyme-linked immunosorbent assays (ELISAs) and other immunoassays to detect HBeAg in blood samples. These assays are crucial for diagnosing acute and chronic HBV infections and monitoring the effectiveness of antiviral therapy.
  2. Research: Recombinant HBeAg is used in research to study the immune response to HBV and to develop new therapeutic strategies. It helps researchers understand how the virus evades the immune system and persists in the host.
  3. Vaccine Development: While HBeAg itself is not used in vaccines, understanding its role in HBV infection has contributed to the development of effective HBV vaccines. The recombinant HBV vaccines currently in use target the hepatitis B surface antigen (HBsAg), another important viral protein.

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