HBV core (1-186)

Hepatitis B Virus Core (1-186 a.a.) Recombinant
Cat. No.
BT9886
Source
Synonyms
Appearance
Purity
HBV Core protein is >90% pure as determined by 10% PAGE (Coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein contains the HBV core immunodominant region amino acids 1-186, and fused to a His tag at N- terminus.

Product Specs

Introduction
Hepatitis B virus (HBV) is one of the few known non-retroviruses that utilizes reverse transcription in its replication cycle, unlike HIV, which is a retrovirus. HBV infects cells by attaching to surface receptors and entering the cell. The viral core particles then travel to the hepatocyte nucleus, where the partially double-stranded, relaxed circular DNA (RC-DNA) is repaired to generate covalently closed circular DNA (cccDNA). This cccDNA serves as a template for cellular RNA polymerase II to transcribe viral genomic and sub-genomic RNAs. Among these, the pregenomic RNA (pgRNA) is specifically packaged into progeny capsids and reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum for envelopment and export or return to the nucleus for conversion to cccDNA.
Description
This recombinant HBV core protein, expressed in E. coli, encompasses amino acids 1-186 of the immunodominant region and carries an N-terminal His tag.
Purity
The purity of the HBV Core protein is greater than 90% as assessed by 10% SDS-PAGE and Coomassie blue staining.
Formulation
The HBV Core protein is supplied in a buffer containing 25mM Tris-HCl (pH 8.0), 1.5mM EDTA, 1.5mM Urea, and 50% glycerol.
Stability
For optimal stability, store the HBV Core protein below -18°C. While the protein remains stable at 4°C for up to 1 week, repeated freeze-thaw cycles should be avoided.
Purification Method
HBV Core protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera HBV-infected individuals.

Product Science Overview

Introduction

The Hepatitis B Virus (HBV) is a significant global health concern, causing both acute and chronic infections that can lead to severe liver diseases, including cirrhosis and hepatocellular carcinoma. The HBV core antigen (HBcAg) is a critical component of the virus, playing a vital role in its replication and assembly. The recombinant form of HBV core antigen, specifically the 1-186 amino acid (a.a.) sequence, is widely used in research and diagnostic applications.

Structure and Function

The HBV core antigen is a structural protein that forms the nucleocapsid of the virus. The nucleocapsid encapsulates the viral DNA and the polymerase enzyme, which are essential for viral replication. The core antigen is composed of 183 to 185 amino acids, depending on the HBV genotype. The recombinant HBV core antigen (1-186 a.a.) includes the entire core protein sequence, ensuring that all functional domains are present.

The core antigen self-assembles into an icosahedral capsid, which is crucial for the virus’s life cycle. The capsid protects the viral genome and facilitates its transport to the nucleus of the host cell. Additionally, the core antigen interacts with various host cell proteins, influencing the virus’s replication and persistence.

Recombinant HBV Core Antigen Production

The recombinant HBV core antigen (1-186 a.a.) is typically produced in Escherichia coli (E. coli) expression systems. The gene encoding the core antigen is cloned into a plasmid vector, which is then introduced into E. coli cells. The bacteria express the core antigen, which is subsequently purified using chromatographic techniques. The recombinant protein is often tagged with a histidine (His) tag to facilitate purification and detection .

Applications in Research and Diagnostics

The recombinant HBV core antigen (1-186 a.a.) is extensively used in various research and diagnostic applications:

  1. Vaccine Development: The core antigen is a target for vaccine development, as it elicits a strong immune response. Recombinant HBV core antigen is used to study the immune response and to develop new vaccine candidates.
  2. Antibody Production: The recombinant protein is used to generate antibodies against HBV. These antibodies are essential for diagnostic assays and therapeutic applications.
  3. Diagnostic Assays: The core antigen is used in enzyme-linked immunosorbent assays (ELISA) and other diagnostic tests to detect HBV infection. The presence of HBcAg in a patient’s serum indicates active viral replication and infection.
  4. Structural Studies: The recombinant core antigen is used in structural biology studies to understand the assembly and function of the HBV capsid. These studies provide insights into the virus’s life cycle and identify potential targets for antiviral drugs.

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